Pregled bibliografske jedinice broj: 697868
Comparison of two commercial enzyme-linked immunosorbent assays for cerebrospinal fluid measurement of amyloid β1–42 and total tau
Comparison of two commercial enzyme-linked immunosorbent assays for cerebrospinal fluid measurement of amyloid β1–42 and total tau // Translational Neuroscience, 4 (2013), 2; 234-240 doi:10.2478/s13380-013-0123-4 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 697868 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Comparison of two commercial enzyme-linked immunosorbent assays for cerebrospinal fluid measurement of amyloid β1–42 and total tau
Autori
Babić, Mirjana ; Vogrinc, Željka ; Diana, Andrea ; Klepac, Nataša ; Borovečki, Fran ; Hof, Patrick R. ; Šimić, Goran
Izvornik
Translational Neuroscience (2081-3856) 4
(2013), 2;
234-240
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
Alzheimer’s disease ; biomarkers ; cerebrospinal fluid
Sažetak
Amyloid β1–42 (Aβ1–42), total tau (t-tau), and phosphorylated tau (p-tau) are the main cerebrospinal fluid (CSF) biomarkers for early diagnosis of Alzheimer’s disease (AD). Detection of AD is critically important in view of the growing number of potential new drugs that may influence the course of the disease in its early phases. However, cut-off levels for these CSF biomarkers have not yet been established. Variability in absolute concentrations of AD biomarkers is high among studies and significant differences were noticed even within the same datasets. Variability in biomarkers levels in these assays may be due to many aspects of operating procedures. Standardization of pre- analytical and analytical procedures in collection, treatment, and storage of CSF samples is crucial because differences in sample handling can drastically influence results. Multicenter studies showed that usage of ELISA kits from different manufacturers also affects outcome. So far only very few studies tested the efficiency of ELISA kits produced by different vendors. In this study, the performance of Innogenetics (Gent, Belgium) and Invitrogen (Camarillo, CA, USA) ELISA kits for t-tau and Aβ1–42 was tested. Passing- Bablok analysis showed significant differences between Invitrogen and Innogenetics ELISA methods, making it impossible to use them interchangeably.
Izvorni jezik
Engleski
Znanstvena područja
Kliničke medicinske znanosti
POVEZANOST RADA
Projekti:
HRZZ-IS-09/16 - Otkrivanje i praćenje bioloških biljega radi rane terapijske intervencije u Alzheimerovoj bolesti (Šimić, Goran, HRZZ ) ( CroRIS)
MZOS-108-1081870-1942 - Fosforilacija tau proteina u razvitku i Alzheimerovoj bolesti (Šimić, Goran, MZOS ) ( CroRIS)
IP-2014-09-4503 - Klostridijski neurotoksini i mozak (BrainTox) (Lackovic, Zdravko, HRZZ - 2014-09) ( CroRIS)
Ustanove:
Medicinski fakultet, Zagreb,
Klinički bolnički centar Zagreb
Profili:
Mirjana Babić Leko
(autor)
Goran Šimić
(autor)
Fran Borovečki
(autor)
Željka Vogrinc
(autor)
Nataša Klepac
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
Uključenost u ostale bibliografske baze podataka::
- BIOSIS Previews (Biological Abstracts)
- EMBASE (Excerpta Medica)