Naslov
Slc26a9—Anion Exchanger, Channel and Na+ Transporter

Autori
Chang, Min-Hwang: Plata, Consuelo ; Zandi-Nejad, Kambiz ; Sinđić, Aleksandra ; Sussman, R. Caroline ; Mercado, Adriana ; Broumand, Vadjista ; Raghuram, Viswanathan ; Mount, B. David B ; Romer, F. Michael

Izvornik
Journal of membrane biology (0022-2631) 228 (2009), 3; 125-140

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
intracellular pH
Cl-
Na+
HCO3

Sažetak
The SLC26 gene family encodes anion transporters with diverse functional attributes: (a) anion exchanger, (b) anion sensor, and (c) anion conductance (likely channel). We have cloned and studied Slc26a9, a paralogue expressed mostly in lung and stomach. Immunohistochemistry shows that Slc26a9 is present at apical and intracellular membranes of lung and stomach epithelia. Using expression in Xenopus laevis oocytes and ion-sensitive microelectrodes, we discovered that Slc26a9 has a novel function not found in any other Slc26 proteins: cation coupling. Intracellular pH and voltage measurements show that Slc26a9 is a nCl--HCO3 - exchanger, suggesting roles in gastric HCl secretion or pulmonary HCO3 - secretion ; Na? electrodes and uptakes reveal that Slc26a9 has a cation dependence. Single-channel measurements indicate that Slc26a9 displays discrete open and closed states. These experiments show that Slc26a9 has three discrete physiological modes: nCl--HCO3 - exchanger, Cl- channel, and Na?-anion cotransporter. Thus, the Slc26a9 transporter channel is uniquely suited for dynamic and tissue-specific physiology or regulation in epithelial tissues.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA