Naslov
PCL-coated hydroxyapatite scaffold derived from cuttlefish bone : in vitro cell culture studies

Autori
Milovac, Dajana ; Gamboa-Martínez, Tatiana C. ; Ivanković, Marica ; Gallego Ferrer, Gloria ; Ivanković, Hrvoje

Izvornik
Materials Science and Engineering C. Materials for Biological Applications (0928-4931) 42 (2014); 264-272

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
cuttlefish bone; poly(ε-caprolactone); hydroxyapatite scaffold; tissue engineering; cell differentiation

Sažetak
In the present study, we examined the potential of using highly porous poly(ε-caprolactone) (PCL)- coated hydroxyapatite (HAp) scaffold derived from cuttlefish bone for bone tissue engineering applications. The cell culture studies were performed in vitro with preosteoblastic MC3T3-E1 cells in static culture conditions. Comparisons were made with uncoated HAp scaffold. The attachment and spreading of preosteoblasts on scaffolds were observed by Live/Dead staining Kit. The cells grown on the HAp/PCL composite scaffold exhibited greater spreading than cells grown on the HAp scaffold. DNA quantification and scanning electron microscopy (SEM) confirmed a good proliferation of cells on the scaffolds. DNA content on the HAp/PCL scaffold was significantly higher compared to porous HAp scaffolds. The amount of collagen synthesis was determined using a hydroxyproline assay. The osteoblastic differentiation of the cells was evaluated by determining alkaline phosphatase (ALP) activity and collagen type I secretion. Furthermore, cell spreading and cell proliferation within scaffolds were observed using a fluorescence microscope.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Kemijsko inženjerstvo

POVEZANOST RADA