Naslov
A study of the inhibition of catalase by dipotassium trioxohydroxytetrafluorotriborate K2[B3O3F4OH]

Autori
Islamović, Safija ; Galić, Borivoj ; Miloš, Mladen ;

Izvornik
Journal of enzyme inhibition and medicinal chemistry (1475-6366) 29 (2014), 5; 744-748

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
boroxine; catalase; drug research; enzyme inhibition

Sažetak
In the development of boronic acid-based enzyme inhibitors as potential pharmaceutical drugs, dipotassium trioxohydroxytetrafluorotriborate K2[B3O3F4OH] was listed as a promising new therapeutic for treatment of different diseases. The catalase-mediated conversion of hydrogen peroxide, in the presence and absence of K2[B3O3F4OH] was studied. The kinetics conformed to the Michaelis–Menten model. Lineweaver–Burk plots were linear and plotted the family of straight lines intersected on the abscissa indicating non-competitive inhibition of the catalase. It appears that in the absence of inhibitor, catalase operates the best at conditions around pH 7.1 and in the presence of K2[B3O3F4OH] the optimum is around pH 6.2. The uncatalyzed reaction of hydrogen peroxide decomposition generally has a value of activation energy of 75 kJ mole−1, whereas catalase, in the absence of inhibitor, lowers the value to 11.2 kJ  mole−1, while in the presence 69 mmoles L−1 of K2[B3O3F4OH] it was 37.8 kJ mole−1.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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