Naslov
European validation of Real-Time PCR method for detection of Salmonella spp. in pork meat

Autori
Delibato, Elisabetta ; Rodriguez Lazaro, David ; Gianfranceschi, Monica ; De Cesare, Alessandra ; Damiano Comin ; Antonietta Gattuso ; Marta Hernandez ; Michele Sonnessa ; Frédérique Pasquali ; Zuzsanna Sreter-Lancz ; María-José Saiz-Abajo ; Javier Pérez-De-Juan ; Javier Butrón ; Prukner-Radovčić, Estella ; Horvatek Tomić, Danijela ; Gro S. Johannessen ; Džiuginta Jakočiūnė ; Olsen, E. John ; Chemaly, Marianne ; Francoise Le Gall ; Patricia González-García ; Antonia Anna Lettini ; Lukač, Maja ; Segolénè Quesne ; Claudia Zampieron ; Paola De Santis ; Sarah Lovari ; Barbara Bertasi ; Enrico Pavoni ; Yolande T.R. Proroga ; Capuano, Federico ; Manfreda, Gerardo ; De Medici, Dario

Izvornik
International journal of food microbiology (0168-1605) 184 (2014); 134-138

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Validation; Salmonella; Real Time PCR; ISO 6579; Pork meat; Artificial contamination

Sažetak
The classical microbiological method for detection of Salmonella spp. requires more than five days for final confirmation, and consequently there is a need for an alternative methodology for detection of this pathogen particularly in those food categories with a short shelf-life. This study presents an international (at European level) ISO 16140-based validation study of a non-proprietary Real-Time PCR-based method that can generate final results the day following sample analysis. It is based on an ISO compatible enrichment coupled to an easy and inexpensive DNA extraction and a consolidated Real-Time PCR assay. Thirteen laboratories from seven European Countries participated to this trial, and pork meat was selected as food model. The limit of detection observed was down to 10 CFU per 25 g of sample, showing excellent concordance and accordance values between samples and laboratories (100%). In addition, excellent values were obtained for relative accuracy, specificity and sensitivity (100%) when the results obtained for the Real-Time PCR-based methods were compared to those of the ISO 6579:2002 standard method. The results of this international trial demonstrate that the evaluated Real-Time PCR-based method represents an excellent alternative to the ISO standard. In fact, it shows an equal and solid performance as well as it reduces dramatically the extent of the analytical process, and can be easily implemented routinely by the Competent Authorities and Food Industry laboratories.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

Napomena
Selection and improving of fit-for-purpose sampling procedures for specific foods and risks (Baseline S.I.).

POVEZANOST RADA