Naslov
Carbohydrate-lectin interaction on graphene-coated surface plasmon resonance (SPR) interfaces

Autori
Penezić, Abra ; Deokar, Geetanjali ; Vignaud, Dominique ; Pichonat, Emmanuelle ; Subramanian, Palaniappan ; Gašparović, Blaženka ; Boukherroub, Rabah ; Szunerits, Sabine

Izvornik
Plasmonics (1557-1955) 9 (2014), 3; 677-683

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
graphene; surface plasmon resonance; mannose; lectins; biosensors

Sažetak
The paper describes the detection of carbohydrate-lectin interaction on graphene-on-metal surface plasmon resonance (SPR) interfaces. Graphene-coated gold-based SPR interfaces were formed through the transfer of large-area graphene grown by chemical vapour deposition (CVD) on polycrystalline Cu foils. The method allowed successful transfer of single and double layered graphene sheets onto the SPR interfaces in a reproducible manner. Functionalization of the graphene interface with mannose was achieved by simple immersion into a mannose aqueous solution (100 mM), resulting in noncovalent -stacking interactions between the aromatic ring structure of graphene and mannose. The utility of the carbohydrate-modified graphene-on-gold interface for the selective and sensitive detection of carbohydrate-lectin interactions was demonstrated using model lectins from Lens culinaris (LC) and Triticum Vulgaris (TV). While LC lectin binds specifically to mannopyranoside units, TV lectin has an affinity for N-acetyl glucosamine and sialic acid residues.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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