Naslov
Biological effects of resin based dental material monomers

Autori
Rajić, Valentina ; Miletić, Ivana ; Želježić, Davor ; Mladinić, Marin ; Anić, Ivica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Journal of Dental Research / - , 2013, 73-73

Skup
46th Meeting od CED-IADR

Mjesto i datum
Firenca, Italija, 04.09.2013. - 07.09.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
monomer ; composite resin material

Sažetak
The objective was to investigate the cytotoxicity and genotoxicity of four resin-based materials, monomers TEGDMA and Bis-GMA in vitro on human lymphocytes and PK 15 cells. The tested materials were Tetric EvoCeram, Tetric EvoFlow, G-aenial and G-aenial Flo. Cytotoxicity was evaluated for every material, non-cured and cured, after four hour and 24 hours. There were two mass concentrations of materials (0, 007g/ml and 0, 013 g/ml) that were subjected to elution in cell culture medium RPMI 1640 for every time period. Genotoxicity was evaluated using micronucleus assay. The cytotoxicity and genotoxicity were also tested on Bis-GMA and TEGDMA, but only on human lymphocytes. Genotoxic effects showed only unpolymerized forms of tested materials, in higher mass concentration. Tetric EvoCeram and Tetric EvoFlow showed the highest genotoxic effect in both tested cell cultures. The cytotoxicity of G-aenial and G-aenial Flo in both cell cultures caused an increase of cells in late apoptosis and necrosis. The results indicate also the cytotoxic effect of Bis-GMA. After four hours cell survival was less than 10%. Surviving cells were in apoptosis and necrosis. TEGDMA caused a significant increase in the number of micronuclei after 24 hours. After four hours TEGDMA caused apoptosis and decrease in the percentage of viable cells at 40%. The results indicate that uncured resin composite components have a cytotoxic and genotoxic potential. Monomers TEGDMA and Bis-GMA induced apoptosis and necrosis

Izvorni jezik
Engleski

Znanstvena područja
Dentalna medicina

POVEZANOST RADA