Naslov
Phenotype, function and development of B cells in NKG2D-deficient mice

Autori
Gulin, Maja ; Wensveen, Felix Martinus ; Jelenčić, Vedrana ; Ožanić, Mateja ; Šantić, Marina ; Polić, Bojan ;

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Godišnji skup Hrvatskog imunološkog društva, Marija Bistrica 2012.

Mjesto i datum
Marija Bistrica, Hrvatska, 05.10.2012. - 07.10.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
NKG2D; B1 cells; B cell developmnt; sepsis

Sažetak
In this study we investigated the role of the NKG2D receptor in the establishment and maintenance of the B cell pool. Flow cytometric analysis revealed perturbances in sizes of different B cell subsets in spleen and peritoneal cavity of NKG2D-deficient mice. We observed two fold reduction of B1 cell compartment size. In correlation with this finding, we found lower levels of IgM antibodies in both cavities but no difference in the sera. After immunization with TI or TD antigens, knock outs show no clear difference in acquiring B cell responses, respectively. Experiments using bone marrow chimeras reconstituted with mixed fetal liver cells of WT and NKG2D-deficient mice showed that in B1a compartment in the peritoneal cavity contains relatively more wild type cells, whereas B2 cells originated more from knock-out donor cells in all investigated organs. The reduced number of B1a cells in NKG2D-/- mice had also functional consequences, as peritoneal infection with Francisella novicida resulted in a higher bacterial load in various organs. We also found that upon induction of sepsis with the caecal ligation and puncture model, knock-out mice were much more susceptible to peritonitis and had a higher mortality rate. Here we report that NKG2D deficiency results in impaired B cell development which leads to lower numbers of B1a cells in the peritoneal cavity, subsequently leading to lower IgM levels. NKG2D-deficient mice are therefore more susceptible to bacterial infections because of the lower levels of natural antibodies.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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