Naslov
Composition and antioxidant activity of aqueous and ethanolic Pelargonium radula extracts

Autori
Petlevski, Roberta ; Flajs, Dubravka ; Kalođera, Zdenka ; Zovko Končić, Marijana

Izvornik
South African journal of botany (0254-6299) 85 (2013); 17-22

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
antioxidant; diabetes; lactate dehydrogenase; Pelargonium radula; polyphenols

Sažetak
Pelargonium radula(Cav.)L'Her is a plant with hypoglycaemic properties originating from South Africa. Since oxidative stress plays a major role in the development of diabetic complications, the aim of this study was to evaluate if P. radula besides its glucose-lowering properties, can have additional beneficial effects on diabetes. For preparation of extracts fresh or dried leaves were extracted at 20 C with either ethanol or water. Since P. radula is commercially cultivated for its essential oil, the residues obtained after steam destillation of essential oil from dry or fresh leaves were also included in the investigation. Content of phenols (total phenols, flavonoids and tannins) was determined in the extracts. Antioxidant activity was established as radical scavenging and chelating activity, reducing power and activity in beta-carotene-linoleic acid assay. To establish possible intracellular effects of the extracts, lactate dehydrogenase (LDH) leakage was determined in Hep G2 cells with glucose induced oxidative stress. All the extracts were found to posses antioxidant activities and were able to reduce LDH leakage from Hep G2 cells. In addition to having excellent extraction yields the residues obtained after steam destillation of essential oil showed the most pronounced antioxidant activity in our study. The results of the present study suggest that P. radula leaf might be useful as complementary therapy in diabetes.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA