Naslov
First evidence of the presence of Multixenobiotic Resistance Mechanism activity in freshwater invasive species, signal crayfish Pacifastacus leniusculus (Dana, 1852)

Autori
Bielen, Ana ; Vladušić, Tomislav ; Kuharić, Nikolina ; Hudina, Sandra ; Lidija, Šver ; Likić Saša ; Bošnjak, Ivana

Izvornik
Periodicum biologorum (0031-5362) 116 (2014), 3; 267-273

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
heavy metals; multixenobiotic resistance mechanism; Pacifastacus leniusculus; signal crayfish

Sažetak
The main goal of this study was to functionally characterize multixenobiotic resistance mechanism defense activity in tissues of the signal crayfish (*Pacifastacus leniusculus*). This invertebrate is one of the most successful crayfish invaders of European freshwaters, extremely diverse and endangered ecosystems. We determined that the optimal time for fluorescent model substrate rhodamine B accumulation in gills and hepatopancreas was 30 minutes. Based on the effect of known inhibitors of multixenobiotic resistance mechanism (5 - 50 µmol l-1 MK571 and 10 - 50 µmol l-1 verapamil), we concluded that multidrug resistance-associated proteins are dominant in gills, and P-glycoprotein-like activity in hepatopancreas. Finally, we detected inhibitory effect of mercury (HgCl2: 10 and 20 µmol l-1) and zinc (ZnCl2: 5 - 20 µmol l-1), known freshwater metal pollutants, on multixenobiotic resistance mechanism activity in gills, and only mercury in hepatopancreas. Therefore, our results for the first time demonstrate the protective relevance of multixenobiotic resistance mechanism efflux activity as important tissue specific defense mechanism in *P. leniusculus

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija

POVEZANOST RADA