Naslov
Evolution of bacterial population: The metabolic engineering of Lactobacillus brevis

Autori
Butorac, Ana ; Cindrić, Mario ; Mrvčić, Jasna ; Bačun-Družina ; Višnja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
The 2th International Symposium "Vera Johanides" / Kniewald ; Zlatko - Zagreb, 2013, 122-122

ISBN
978-953-7076-21-4

Skup
The 2th International Symposium "Vera Johanides"

Mjesto i datum
Zagreb, Hrvatska, 10.06.2013. - 11.06.2013

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
Lactobacillus; evolution; long term starvation

Sažetak
Lactic acid bacteria in particular Lactobacilli are widely used in food and pharmaceutical industries. Lactobacillus brevis occupies a diverse range of environmental niches and has various metabolic capacities. Its genomic islands are consisted of numerous functional gene cassettes, especially for carbohydrates utilization, which can be mobilized, shuffled, deleted or substituted in response to niche demands. We used laboratory evolution experiments to explore bacterial adaptation under well-defined selective pressure of prolonged stationary phase growth. Genomics analyses of L. brevis after long term-starvation indicated that cells underwent chromosomal rearrangements and plasmid loss. Furthermore, proteomics analysis revealed the key differences in metabolic pathways responsible for the existence of cells. After prolonged starvation of cells the numerous proteins engaged in glucose and amino-acid catabolizing pathways, glycerolipid metabolizing pathways, and stress-response mechanisms were all differentially expressed. Metabolomics analysis indicated that the studied cells produce less lactic acid in comparison to parental cells. The understanding of experimental evolution, such as the long-term adaptation, may facilitate new strategies for development of cell lines with advantageous traits.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA