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Pregled bibliografske jedinice broj: 670909

Monitoring mitochondrial electron fluxes using NAD(P)H-flavoprotein fluorometry reveals complex action of isoflurane on cardiomyocytes

Sedlić, Filip; Pravdić, Danijel; Hirata, N.; Mio, Y.; Šepac, Ana; Camara, A.K.; Wakatsuki, Tetsuro; Bošnjak, Željko; Bienengraeber, Martin
Monitoring mitochondrial electron fluxes using NAD(P)H-flavoprotein fluorometry reveals complex action of isoflurane on cardiomyocytes // Biochimica et biophysica acta. Bioenergetics, 1797 (2010), 10; 1749-1758 doi:10.1016/j.bbabio.2010.07.009 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 670909 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Monitoring mitochondrial electron fluxes using NAD(P)H-flavoprotein fluorometry reveals complex action of isoflurane on cardiomyocytes

Autori
Sedlić, Filip ; Pravdić, Danijel ; Hirata, N. ; Mio, Y. ; Šepac, Ana ; Camara, A.K. ; Wakatsuki, Tetsuro ; Bošnjak, Željko ; Bienengraeber, Martin

Izvornik
Biochimica et biophysica acta. Bioenergetics (0005-2728) 1797 (2010), 10; 1749-1758

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
NAD(P)H fluorometry; flavoprotein fluorometry; electron transport chain; cardiomyocyte; reactive oxygen species; isoflurane

Sažetak
Mitochondrial bioenergetic studies mostly rely on isolated mitochondria thus excluding the regulatory role of other cellular compartments important for the overall mitochondrial function. In intact cardiomyocytes, we followed the dynamics of electron fluxes along specific sites of the electron transport chain (ETC) by simultaneous detection of NAD(P)H and flavoprotein (FP) fluorescence intensities using a laser-scanning confocal microscope. This method was used to delineate the effects of isoflurane, a volatile anesthetic and cardioprotective agent, on the ETC. Comparison to the effects of well-characterized ETC inhibitors and uncoupling agent revealed two distinct effects of isoflurane: uncoupling-induced mitochondrial depolarization and inhibition of ETC at the level of complex I. In correlation, oxygen consumption measurements in cardiomyocytes confirmed a dose-dependent, dual effect of isoflurane, and in isolated mitochondria an obstruction of the ETC primarily at the level of complex I. These effects are likely responsible for the reported mild stimulation of mitochondrial reactive oxygen species (ROS) production required for the cardioprotective effects of isoflurane. In conclusion, isoflurane exhibits complex effects on the ETC in intact cardiomyocytes, altering its electron fluxes, and thereby enhancing ROS production. The NAD(P)H-FP fluorometry is a useful method for exploring the effect of drugs on mitochondria and identifying their specific sites of action within the ETC of intact cardiomyocytes.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA

Profili:

Avatar Url Ana Šepac (autor)

Avatar Url Filip Sedlić (autor)

Poveznice na cjeloviti tekst rada:

Pristup cjelovitom tekstu rada doi

Citiraj ovu publikaciju:

Sedlić, Filip; Pravdić, Danijel; Hirata, N.; Mio, Y.; Šepac, Ana; Camara, A.K.; Wakatsuki, Tetsuro; Bošnjak, Željko; Bienengraeber, Martin
Monitoring mitochondrial electron fluxes using NAD(P)H-flavoprotein fluorometry reveals complex action of isoflurane on cardiomyocytes // Biochimica et biophysica acta. Bioenergetics, 1797 (2010), 10; 1749-1758 doi:10.1016/j.bbabio.2010.07.009 (međunarodna recenzija, članak, znanstveni)
Sedlić, F., Pravdić, D., Hirata, N., Mio, Y., Šepac, A., Camara, A., Wakatsuki, T., Bošnjak, Ž. & Bienengraeber, M. (2010) Monitoring mitochondrial electron fluxes using NAD(P)H-flavoprotein fluorometry reveals complex action of isoflurane on cardiomyocytes. Biochimica et biophysica acta. Bioenergetics, 1797 (10), 1749-1758 doi:10.1016/j.bbabio.2010.07.009.
@article{article, author = {Sedli\'{c}, Filip and Pravdi\'{c}, Danijel and Hirata, N. and Mio, Y. and \v{S}epac, Ana and Camara, A.K. and Wakatsuki, Tetsuro and Bo\v{s}njak, \v{Z}eljko and Bienengraeber, Martin}, year = {2010}, pages = {1749-1758}, DOI = {10.1016/j.bbabio.2010.07.009}, keywords = {NAD(P)H fluorometry, flavoprotein fluorometry, electron transport chain, cardiomyocyte, reactive oxygen species, isoflurane}, journal = {Biochimica et biophysica acta. Bioenergetics}, doi = {10.1016/j.bbabio.2010.07.009}, volume = {1797}, number = {10}, issn = {0005-2728}, title = {Monitoring mitochondrial electron fluxes using NAD(P)H-flavoprotein fluorometry reveals complex action of isoflurane on cardiomyocytes}, keyword = {NAD(P)H fluorometry, flavoprotein fluorometry, electron transport chain, cardiomyocyte, reactive oxygen species, isoflurane} }
@article{article, author = {Sedli\'{c}, Filip and Pravdi\'{c}, Danijel and Hirata, N. and Mio, Y. and \v{S}epac, Ana and Camara, A.K. and Wakatsuki, Tetsuro and Bo\v{s}njak, \v{Z}eljko and Bienengraeber, Martin}, year = {2010}, pages = {1749-1758}, DOI = {10.1016/j.bbabio.2010.07.009}, keywords = {NAD(P)H fluorometry, flavoprotein fluorometry, electron transport chain, cardiomyocyte, reactive oxygen species, isoflurane}, journal = {Biochimica et biophysica acta. Bioenergetics}, doi = {10.1016/j.bbabio.2010.07.009}, volume = {1797}, number = {10}, issn = {0005-2728}, title = {Monitoring mitochondrial electron fluxes using NAD(P)H-flavoprotein fluorometry reveals complex action of isoflurane on cardiomyocytes}, keyword = {NAD(P)H fluorometry, flavoprotein fluorometry, electron transport chain, cardiomyocyte, reactive oxygen species, isoflurane} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus

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