Naslov
Proteomics profiling of keratocystic odontogenic tumours reveals AIDA as novel biomarker candidate

Autori
Ivanišević Malčić, Ana ; Breen, Lucas ; Josić, Djuro ; Jukić Krmek, Silvana ; Džombeta, Tihana ; Matijević , Jurica ; Grgurević, Lovro ; Pavelić, Krešimir ; Krušlin, Božo ; Kraljević Pavelić, Sandra

Izvornik
Journal of oral pathology & medicine (0904-2512) 44 (2014), 5; 367-377

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
AIDA; biomarkers; immunohistochemistry; keratocystic odontogenic tumor; mass

Sažetak
Background. Keratocystic odontogenic tumor (KCOT) is a benign, yet aggressive odontogenic tumor. Herein, proteome analysis of KCOT lesions in comparison with control patient-matched tissue unaffected by the disease and with inflammatory odontogenic cysts, namely radicular cysts is presented. Methods. For the proteomics profiling, two complementary proteomics techniques MALDI-MS/MS and LC-ESI-MS/MS were employed. Potential candidate biomarkers were validated by immunohistochemistry. Results. More than 43 proteins were found to be differentially expressed or up-regulated in KCOT lesions in comparison with patient-matched unaffected oral mucosa. These proteins bear important biological functions and are involved in cell proliferation, cytoskeletal re-organization, transcription, cellular motility and apoptosis. In particular, a number of differentially expressed proteins participate in autocrine regulation and signalization within JNK and p38 MAPK signaling pathways. Conclusions. Immunohistochemical validation of chosen putative biomarkers revealed axin interaction partner and dorsalization- antagonist (AIDA), known as a protein that blocks activation of JNK signalling pathway , as a differential biomarker for KCOT lesions on an independent cohort of KCOT tissue samples in comparison with most prevalet intraoseal lesions inflammatory odontogenic cysts.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Dentalna medicina

POVEZANOST RADA