Naslov
Synergism Between Propolis and Hyperthermal Intraperitoneal Chemotherapy with Cisplatin on Ehrlich Ascites Tumor in Mice

Autori
Oršolić, Nada ; Car, Nikola ; Lisičić, Duje ; Benković, Vesna ; Horvat Knežević, Anica ; Đikić, Domagoj ; Petrik, Jozsef

Izvornik
Journal of pharmaceutical sciences (0022-3549) 102 (2013), 12; 4395-4405

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
hyperthermia; chemotherapy; immunomodulation; propolis; cisplatin; Ehrlich ascites tumorp; cancer chemopreveention; immunotherapy; natural products; toxicity; food interaction; hyperthemia; cisplatin; ehrlich ascites tumor

Sažetak
We investigated antitumor, genotoxic, chemopreventive, and immunostimulative effects of local chemoimmunotherapy and hyperthermal intraperitoneal chemotherapy (HIPEC) in a mouse-bearing Ehrlich ascites tumor (EAT). Mice were treated with water-soluble derivative of propolis (WSDP) at a dose of 50 mg kg−1, 7 and 3 days before implantation of EAT cells, whereas cisplatin (5 or 10 mg kg−1) was injected 3 days after implantation of EAT cells at 37°C and 43°C. The following variables were analyzed: the total number of cells, differential count of the cells present in the peritoneal cavity, functional activity of macrophages, comet assay, and micronucleus assay. The combination of WSDP + CIS 5 mg kg−1 at 37°C resulted in tumor growth inhibition and increased the survival of mice by additional 115.25%. WSDP with HIPEC increased the survival of mice by additional 160.3% as compared with HIPEC. WSDP reduced cisplatin toxic and genotoxic effect to normal cells without affecting cisplatin cytotoxicity on EAT cells. In addition, WSDP with HIPEC increased the cytotoxic actions of macrophages to tumor cells. Water-soluble derivative of propolis increases macrophage activity and sensitivity of tumor cells to HIPEC and reduces cisplatin toxicity to normal cells.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Farmacija

POVEZANOST RADA