Naslov
Mice deficient in p52/NF-κB as a model for studying prion disease

Autori
Poljak, Ljiljana

Izvornik
Translational Neuroscience (2081-3856) 3 (2012), 2; 167-169

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, kratko priopcenje, znanstveni

Ključne riječi
p52 knockout mice ; Prion disease ; Follicular dendritic cells development

Sažetak
A tight functional link between the central nervous and peripheral immune system closely related to the activity of NF-kappa B transcription factor has been evidenced in numerous brain pathological conditions. One of the most instructive examples is a prion neuroinvasion and the development of transmissible spongioform encephalopathy (TSE) or prion diseases. The failure of peripherally administrated prions to elicit disease in immune- deficient mice indicates that preserved organ microarchitecture is crucial for TSE pathogenesis and it seems to go through topographic relationships between follicular dendritic cells and sympathetic endings in lymphoid organs. New insight into the molecular requirements for follicular dendritic cell (FDC) development in NF-kappa B p52 deficient mice highlights the role of NF-kappa B transcription factor in prion disease progression. However, adoptive transfer of wild type bone marrow cells (NF-kappa B +/+ background) into p52 deficient animals (NF-kappa B -/- background) does not correct the FDC defect. RT-PCR analysis of the expression level of mRNA for LTa, LTb, TNFRI and BLC of the splenic cells from p52/NF-kappa B mutant/wt BM chimera did not reveal major changes compared to wt/wt BM chimeras, indicating that their normal expression within splenic tissue does not assure for complete restoration of the FDC network in p52/NF-kappa B mutant. This implies that the distance between FDCs and splenic nerve terminals around the central arteriole is longer and could interfere with prion spreading in lymphoid organs prior to neuroinvasion. In light of the fact that prion neuroinvasion highly depends on the physical distance between sympathetic nerve endings and FDCs dendritic extensions, it is not hard to imagine that this could be the way to approach the problem of prion diseases development.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA