Naslov
Hydroxytyrosol expresses antifungal activity in vitro

Autori
Zorić, Nataša ; Horvat, Igor ; Kopjar, Nevenka ; Vučemilović, Ante ; Kremer, Dario ; Tomić, Siniša ; Kosalec, Ivan

Izvornik
Current drug targets (1389-4501) 14 (2013); 992-998

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
antifungal activity; Candida albicans; cell surface hydrophobicity; germ-tube formation hydroxytyrosol; virulence factors; olive leaf

Sažetak
Hydroxytyrosol (HT) is a potent antioxidant found in olive oil and leaves. Using several in vitro approaches, we tested antifungal activity of HT. HT showed broad spectrum of antifungal activity against medically important yeasts and dermatophyte strains with MIC values ranging between 97.6 μgml-1 and 6.25 mgml-1. The antimicrobial activity of HT was also tested using the time-kill methodology. Below the MIC value, HT showed potent damage of cell wall of Candida albicans ATCC 10231 using fluorescent dye-exclusion method. At the subinhibitory concentration, HT also influenced dimorphic transition of C. albicans indicating that HT is inhibitor of germ-tube formation as one of the most important virulence factor of C. albicans. Furthermore, HT showed disturbances in cell surface hydrophobicity (CSH) of C. albicans. The in vitro results indicate that HT caused significant cell wall damage and changes in CSH as well as inhibition of germ-tube formation as virulence factor of C. albicans. The study indicates that HT has a considerable in vitro antifungal activity against medically important yeasts.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

POVEZANOST RADA