Naslov
Soluble receptors and monoclonal antibodies to TNF-alpha modulate differently monocyte adhesion molecule expression in rheumatoid arthritis

Autori
Čikeš Čulić, Vedrana ; Tičinović Kurir, Tina ; Martinić, Roko ; Marasović Krstulović, Daniela ; Božić, Joško ; Martinović Kaliterna, Dušanka ; Perković, Dijana ; Markotić, Anita

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Skup
10th Congress of the Croatian Society of Biochemistry and Molecular Biology

Mjesto i datum
Opatija, Hrvatska, 15.09.2010. - 18.09.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
soluble receptors; monoclonal antibody; TNF-alpha; monocyte; adhesion molecules; rheumatoid arthritis

Sažetak
The discovery that the macrophage-derived proinflammatory cytokine tumour necrosis factor alpha (TNF-alpha) plays a central role in the pathogenesis of rheumatoid arthritis (RA) led to the introduction of new biologic, anti-TNF-alpha drugs. TNF-alpha inhibitors are either soluble receptors (etanercept) or monoclonal antibodies (infliximab). Infliximab is described as more immunogenic, with higher cytotoxicity and cellular lysis. RA is a systemic autoimmune disease characterized by infiltration of T-cells and monocytes into the synovium and proliferation of synoviocytes. Leukocyte ingress into the synovium occurs by leukocyte adhesion to endothelial cells and then by transendothelial migration. Adhesion molecules on leukocytes are elevated in RA. Laboratory tests have not yet evaluated the influence of different biologic therapy in RA upon leukocyte adhesion molecule expression. In this study, we aimed to compare the TNF-alpha inhibitory effects of etanercept and infliximab upon leukocyte adhesion molecules. Leukocytes were stained with monoclonal antibodies against leukocyte function antigen-1 (LFA-1 ; CD11a), intercellular adhesion molecule-1 (ICAM-1 ; CD54), very late activation antigen-4 (VLA-4 ; CD49d), hyaluronic acid receptor (CD44), and MHC-class II (HLA-DR) and determined by flow cytometry. Lymphocyte, monocyte and granulocyte subsets were identified by gating analysis. Significant differences in adhesion molecule expression between etanercept and infliximab treated patients were detected only for monocyte subpopulations. Infliximab decreased two fold the proportion of CD54+ HLA-DR+ (p=0.001) and increased four fold HLA-DR- monocyte subpopulation (p=0.004). In conclusion, we propose HLA-DR and CD54 monocyte expression determination as a sensitive tool for evaluation of efficacy of distinct TNF-alpha inhibitor therapy.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita, Farmacija

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