Pregled bibliografske jedinice broj: 657965
Escherichia coli LeuRS CP1 domain operates to exclude norvaline from the cellular proteome
Escherichia coli LeuRS CP1 domain operates to exclude norvaline from the cellular proteome // PRIME-XS Workshop on Phosphopeptide enrichment, labeling strategies and proteomics data analysis
Hrvatska, 2013. (poster, nije recenziran, neobjavljeni rad, znanstveni)
CROSBI ID: 657965 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Escherichia coli LeuRS CP1 domain operates to exclude norvaline from the cellular proteome
Autori
Cvetešić, Nevena ; Gruić-Sovulj, Ita
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni
Skup
PRIME-XS Workshop on Phosphopeptide enrichment, labeling strategies and proteomics data analysis
Mjesto i datum
Hrvatska, 22.10.2013. - 23.10.2013
Vrsta sudjelovanja
Poster
Vrsta recenzije
Nije recenziran
Ključne riječi
leucyl-tRNA synthetase; proofreading; isoleucine; norvaline
Sažetak
LeuRS efficiently activates and transfers norvaline to tRNALeu and thus relies exclusively on post-transfer editing to eliminate norvaline from the genetic code. In sharp contrast, high level of discrimination against isoleucine is achieved in the synthetic reaction, thereby obviating the need for post- transfer editing. CP1 domain is capable of rapid hydrolysis of both Ile- and Nva-tRNALeu. In spite of slow transfer of isoleucyl moiety to tRNALeu, LeuRS does not display tRNA dependent pre- transfer editing. The main function of E. coli LeuRS CP1-editing domain is to exclude norvaline from the cellular proteome.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Biologija
POVEZANOST RADA
Projekti:
119-0982913-1358 - Strukturna raznolikost seril-tRNA sintetaza i točnost biosinteze proteina (Rokov Plavec, Jasmina; Weygand Đurašević, Ivana, MZOS ) ( CroRIS)
Ustanove:
Prirodoslovno-matematički fakultet, Zagreb
