Naslov
In vitro inhibition of blood cholinesterase activities from cattle by triazole fungicides

Autori
Kolesárová, Viera ; Šinko, Goran ; Šiviková, Katarína ; Dianovský, Ján

Izvornik
Caryologia (0008-7114) 66 (2013), 4; 346-350

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
cattle; cholinesterase; exposure; inhibition; tebuconazole-based fungicides

Sažetak
The changes in cattle acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities after exposure to tebuconazole-based fungicides (Orius and Prosaro®) in vitro were determined spectrophotometrically according to the Ellman method. The fungicides induced the decrease in AChE and BChE levels in a dose-dependent manner. Statistically significant inhibition of erythrocyte AChE levels was observed in the concentration range from 150 to 300 μg ml−1 (p < 0.001) for Orius and from 100 to 300 μg ml−1 for Prosaro® (p < 0.01 and p < 0.001, respectively). Levels of BChE activity were more reduced for the fungicide Orius (40–50%) than for Prosaro®. Statistical significance in the inhibition of BChE activity was found after exposure of cattle plasma to both fungicides at concentrations starting from 150 μg ml−1 (p < 0.001). Our results indicate that tebuconazole-based fungicides are able to reduce cattle AChE and BChE activities.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Veterinarska medicina

POVEZANOST RADA