Naslov
Dijagnostika rikecija iz humanih i animalnih uzoraka = Diagnostics of rickettsiae from human and animal samples
(Diagnostics of rickettsiae from human and animal samples)

Autori
Svoboda, Petra ; Lukas, Davorka ; Margaletić, Josip ; Markotić, Alemka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
10. hrvatski kongres kliničke mikrobiologije (CROCMID 2013) i 7. hrvatski kongres o infektivnim bolestima : Knjiga sažetaka = abstract book / Bradarić, Nikola ; Tambić Andrašević, Arjana - : Hrvatski liječnički zbor ; Hrvatsko društvo za mikrobiologiju ; Hrvatsko društvo za infektivne bolesti, 2013, 200-201

Skup
Hrvatski kongres kliničke mikrobiologije (10 ; 2013) ; Hrvatski kongres o infektivnim bolestima (7 ; 2013)

Mjesto i datum
Rovinj, Hrvatska, 24.10.2013. - 27.10.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
molekularna dijagnostika; vanjska membrana proteina B gena; rikecija; Rickettsia africae; glodavci
(molecular diagnostics; outer membrane protein B gene; rickettsiae; Rickettsia africae; rodents)

Sažetak
Rickettsia are gram-negative, pleomorphic, obligate intracellular bacteria transmitted by arthropods, causing human diseases. Due to unspecific clinical symptoms, the disease in humans can be misdiagnosed and undetected. Rodents are suggested reservoir hosts for certain rickettsiae. The most important diagnostic methods for rickettsiae are still serological: immunofluorescence, ELISA, western blot. However, differentiating rickettsial species by serology is difficult due to cross-reactivity between the species (owing to shared protein epitopes and lipopolysaccharides). So, molecular diagnostics of rickettsiae is becoming more important, although taking adequate human samples for molecular diagnostics is time limited due to administering antibiotic therapy and short bacteremia. With the newly established molecular diagnostics at the University Hospital for Infectious Diseases "Dr. Fran Mihaljević" in Zagreb, we detected Rickettsia spp. in human and animal samples. Samples were taken from a patient returning from a trip to Africa and 77 tissue samples of small wild rodents from three different localities in Croatia (Lipovljani, Plitvice, Medvednica). A conventional screening PCR targeting the Rickettsia spp. partial outer membrane protein B gene (ompB) was performed for all samples. The positive result was obtained from the patient’s wound swab sample. Sequencing confirmed that the infection in the patient was caused by Rickettsia africae. Rickettsia spp. was demonstrated in 1.3% of total 77 rodent samples. Given the increasing number of tourists in Croatia and Croatian citizens traveling to Africa, it is necessary to include rickettsioses in the differential diagnosis for the patients returning from a trip to Africa, proving wound swabs to be adequate sample source for the detection of the pathogen. Further studies are needed to determine the significance of small rodents as reservoirs for rickettsiae in different areas in Croatia.

Izvorni jezik
Hrvatski, engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti, Šumarstvo

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