Naslov
DNA damage and apoptosis in mussel Mytilus galloprovincialis

Autori
Mičić, Milena ; Bihari, Nevenka ; Jakšić, Željko ; Mueller, Werner E.G. ; Batel, Renato

Izvornik
Marine Environmental Research (0166-445X) 53 (2002); 243-262

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DNA strand breaks; benzo(a)pyrene; 4-nitroquinoline-N-oxide; tributyltin; mussel; apoptosis; alkaline elution

Sažetak
The effects of known model substances (4-nitroquinoline-N-oxide, benzo(a)pyrene, teniposide, etoposide, cycloheximide, tributyltin) on human cells (FLC, HL-60) and on mussels were investigated. The correlations between formation of DNA strand breaks and DNA fragmentation characteristic for process of apoptosis were estimated. Strand breaks induced by 4-nitroquinoline-N-oxide and benzo(a)pyrene did not correlate with DNA fragmentation detected in the process of apoptosis. Induction of internucleosomal DNA fragmentation in HL-60 cells was found to be initiated by teniposide, etoposide and tributyltin, while in gills of mussel this was detected only with tributyltin. Neither high molecular weight DNA nor internucleosomal DNA fragmentation fragmentation was observed in haemocytes of mussels. Levels of DNA strand breaks in natural mussel populations, living at locations under the influence of urban and industrial wastes, do not mirror the apoptotic processes.

Izvorni jezik
Engleski

Znanstvena područja
Geologija

POVEZANOST RADA