Naslov
NADH oxidation in a microreactor catalyzed by ADH immobilized on γ-Fe2O3 nanoparticles

Autori
Šalić, Anita ; Pindrić Katarina ; Hojnik Podrepšek, Gordana ; Leitgeb, Maja ; Zelić, Bruno

Izvornik
Green Processing and Synthesis (2191-9542) 2 (2013), 6; 569-578

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
maghemite nanoparticle ; alcohol dehydrogenase ; enzyme immobilization ; coenzyme regeneration ; microreactor

Sažetak
A new concept of nicotinamide adenine dinucleotide hydrate (NADH) oxidation which combines advantages of the microreactor technology with the advantages of magnetic nanoparticles (MNPs) application is developed. Acetaldehyde was used as a substrate for the NADH regeneration process while the reaction was performed in a batch reactor and in a microreactor using alcohol dehydrogenase (ADH)- loaded MNPs. Three different microreactor systems with MNPs were studied, two with stationary MNPs trapped on the inner surface of microchannel by permanent magnetic field and one where the MNPs actively move across the channel (movement inside microchannel allowed by oscillating magnetic field). In reactor system with oscillating magnetic field and actively moving ADH-loaded MNPs 100% NADH conversion was achieved for residence time of just 2 min.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo

Napomena
This research was financially supported through the Croatian Science Foundation and CEEPUS SI-0708 Scholarship programme.

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