Naslov
Changes in cardiac innervation during maturation in long-term diabetes

Autori
Baković, Marija ; Jurić Paić, Marina ; Zdrilić, Elena ; Vukojević, Katarina ; Ferhatović, Lejla ; Marin, Ana ; Filipović, Natalija ; Grković, Ivica ; Puljak, Livia

Izvornik
Experimental gerontology (0531-5565) 48 (2013), 12; 1473-1478

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
diabetes mellitus ; innervation ; PGP 9.5 ; neuropathy

Sažetak
Diabetic autonomic neuropathy being a common complication of diabetes mellitus (DM) is related to an increased risk of cardiovascular mortality. However, mechanisms underlying changes of innervations density in affected hearts remain insufficiently understood. Hence, the aim of this study was to describe quantitative changes of intra-myocardial nerve terminals in hearts of diabetic rats of various ages. Male Sprague-Dawley rats were injected with 55 mg/kg streptozotocin (STZ) (DM group) or with citrate buffer (control). After 2 weeks, 2 months, 6 months and or 12 months, sections of their heart s were analyzed in five areas – left ventricle, interventricular septum, right ventricle, anterior and posterior wall. Nerve fibers were visualised immunohistochemicaly, using antibody against a general neuronal marker, protein gene product 9.5 (PGP 9.5). Significant increase in nerve fibers was observed 2 weeks and 2 months after diabetes induction, followed by a decrease at 6 months and again increase at 12 months was observed in both control and diabetic rats. Significant difference between control and diabetic rats was visible after 2 weeks and 2 months, with diabetic rats exhibiting significantly more nerve fibers. There were no consistent differences in quantity of nerve fibers in different areas of the heart within a particular age-related group of animals. In conclusion, cardiac innervation undergoes dynamic changes both in control and in diabetic rats, with a time-dependent significant increase in neuronal fiber density in diabetic animals. This novel information may contribute to our understanding of pathophysiological changes associated with diabetic cardiac neuropathy.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA