Pregled bibliografske jedinice broj: 649503
Improvement of pBBR1MCS plasmids, a very useful series of broad-host-range cloning vectors
Improvement of pBBR1MCS plasmids, a very useful series of broad-host-range cloning vectors // Plasmid, 70 (2013), 2; 263-267 doi:10.1016/j.plasmid.2013.04.001 (međunarodna recenzija, kratko priopcenje, znanstveni)
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Naslov
Improvement of pBBR1MCS plasmids, a very useful series of broad-host-range cloning vectors
Autori
Obranić, Sonja ; Babić, Fedora ; Maravić-Vlahoviček, Gordana
Izvornik
Plasmid (0147-619X) 70
(2013), 2;
263-267
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, kratko priopcenje, znanstveni
Ključne riječi
pBBR1MCS vectors; site-directed mutagenesis; NdeI site; pBBR1MCS_START series
Sažetak
PBBR1MCS vectors are small in size, contain unique cloning sites within the lacZα gene, and are mobilizable and compatible with various plasmid incompatibility groups. We cloned four genes for aminoglycoside resistance methyltransferases from the Arm and Kam families into pBBR1MCS-3 and expressed them in Escherichia coli. The activity of two of these enzymes was impaired because of the fusion with the first 20 amino acids of the β-galactosidase α-peptide derived from the pBBR1MCS-3 vector. In order to overcome this problem, we introduced by site-directed mutagenesis a new NdeI restriction site into pBBR1MCS-3 to generate a start codon directly at the beginning of lacZα gene. We modified the pBBR1MCS-2, 4 and 5 plasmids in the same manner and obtained the enhanced pBBR1MCS_START vector series that retains all the useful features of the previous vectors, but eliminates the unknown effect of the fusion with the β-galactosidase α-peptide.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Farmacija
POVEZANOST RADA
Projekti:
006-0982913-1219 - Molekularne osnove djelovanja antibiotika i mehanizmi bakterijske rezistencije (Maravić Vlahoviček, Gordana, MZOS ) ( CroRIS)
Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE