Naslov
GSTP1, GSTM1 and GSTT1 genetic polymorphisms and total serum GST concentration in stable male COPD

Autori
Žuntar, Irena ; Petlevski, Roberta ; Dodig, Slavica ; Popović-Grle, Sanja

Izvornik
Acta pharmaceutica (1330-0075) 64 (2014); 117-129

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
glutathione S-transferases (GST); polymorphism; COPD; alpha-1-antitrypsin; lactate dehydrogenase

Sažetak
The aim of this study was to test the hypothesis that glutathione-S-transferase (GST) genotypes were associated with COPD. GSTP1, GSTM1 and GSTT1 genotypes were determined by DNA methods and GST activity spectrophotometrically in older male Caucasian Croats (non-smokers, ex-smokers, and smokers) with stable COPD (n=30) and sex/age matched controls (n=60). The distribution of GSTP1 genotypes and alleles in controls vs. COPD showed statistical difference (p<0.05). The odds ratio of CC/CT+TT (wild type GSTP1 exon 6 vs. joint heterozygous and mutant homozygous GSTP1 exon 6) was 10.000 and statistically different (p=0.002). By this study, the GSTP1 mutant genotype of exon 5 (GG), and also GSTP1 mutant and heterozygous genotypes of exon 6 (TT and CT) were suggested to be a genetic contributors to COPD susceptibility. Null GSTM1, null GSTT1 and the joint GSTM1/GSTT1 null genotypes were not disease-associated. Serum GST was not associated with GST genotypes and COPD as well as smoking history in our study subjects. Conclusions reviled from the study should be furthermore supported and clarified by studies with larger sample sizes.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Javno zdravstvo i zdravstvena zaštita, Farmacija

POVEZANOST RADA