Naslov
Application of LAMP method for detection and quantification of Newcastle disease virus

Autori
Kos, Dragana ; Jurišić, Ivana ; Nedeljković, Gordana ; Gottstein, Željko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts / Horvatek Tomić, Danijela ; Severin, Krešimir ; Slavica, Alen - Zagreb : Veterinarski fakultet Sveučilišta u Zagrebu, 2013, 57-57

Skup
The 5th International Congress "Veterinary Science and Profession"

Mjesto i datum
Zagreb, Hrvatska, 03.10.2013. - 04.10.2013

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Newcastle disease virus; qRT-LAMP; qRT-PCR; absolute quantification

Sažetak
Newcastle disease is one of highly contagious viral infectious diseases of domestic poultry and wild birds with a high morbidity and mortality and expensive control, suppression and eradication measures. To apply appropriate measures on time, due to its devastating effects on poultry industry, it needs rapid, simple and reliable diagnostic method. The aim of this work is to explore the possibility of using loop-mediated isothermal amplification (LAMP) in detection and absolute quantification of Newcastle disease virus in clinical samples. Reverse transcription of viral RNA, amplification and detection of target sequences was performed in one step at a constant temperature during the reaction in a RealTime PCR device. Real time monitoring of LAMP has shown positive reaction in 6, 5 to 14 minutes and enabled the approximate absolute quantification of initial viral nucleic acid concentration of a sample based on the standard curve. RT-LAMP has proved itself to be a simple, rapid, specific and sensitive method of nucleic acid amplification as well as quantification.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita, Veterinarska medicina

POVEZANOST RADA