Assessment of DNA damage and lipid peroxidation in diabetic mice : effects of propolis and epigallocatechin gallate (EGCG)

Oršolić, Nada; Sirovina, Damir; Gajski, Goran; Garaj-Vrhovac, Vera; Jazvinšćak Jembrek, Maja; Kosalec, Ivan

doi:10.1016/j.mrgentox.2013.04.022

Pregled bibliografske jedinice broj: 646516

Assessment of DNA damage and lipid peroxidation in diabetic mice : effects of propolis and epigallocatechin gallate (EGCG)

Oršolić, Nada; Sirovina, Damir; Gajski, Goran; Garaj-Vrhovac, Vera; Jazvinšćak Jembrek, Maja; Kosalec, Ivan

Assessment of DNA damage and lipid peroxidation in diabetic mice : effects of propolis and epigallocatechin gallate (EGCG) // Mutation research. Genetic toxicology and environmental mutagenesis, 757 (2013), 1; 36-44 doi:10.1016/j.mrgentox.2013.04.022 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 646516 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Assessment of DNA damage and lipid peroxidation in diabetic mice : effects of propolis and epigallocatechin gallate (EGCG)

Autori
Oršolić, Nada ; Sirovina, Damir ; Gajski, Goran ; Garaj-Vrhovac, Vera ; Jazvinšćak Jembrek, Maja ; Kosalec, Ivan

Izvornik
Mutation research. Genetic toxicology and environmental mutagenesis (1383-5718) 757 (2013), 1; 36-44

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
propolis; EGCG; alloxan; diabetes; DNA damage; lipid peroxidation

Sažetak
There is growing recognition that polyphenolic compounds present in many plants and natural products may have beneficial effects on human health. Propolis - a substance produced by honeybees - and catechins in tea, in particular (-)-epigallocatechin gallate (EGCG), are strong antioxidants that appear to have anti-obesity and anti-diabetic effects. The present study was designed to elucidate the anti-diabetic effect of the water-soluble derivative of propolis (WSDP), which contains phenolic acids as the main compounds, and EGCG in alloxan-induced (75mg/kg, iv) diabetes in mice. Intraperitoneal administration of EGCG or propolis at doses of 50mg/kg body weight (bw) to diabetic mice for a period of 7 days resulted in a significant increase in body weight and in haematological/immunological blood parameters, as well as in 100% survival of the mice. A significant decrease in lipid peroxidation in liver, kidney and brain tissue was also observed in diabetic mice treated with these two agents. Additionally, EGCG and propolis clearly reduced DNA damage in peripheral lymphocytes of diabetic mice. Our studies demonstrate the anti-oxidative and anti-inflammatory potential of WSDP and EGCG, which could exert beneficial effects against diabetes and the associated consequences of free-radical formation in kidney, liver, spleen and brain tissue. The results suggest that dietary supplementation with WSDP or EGCG could potentially contribute to nutritional strategies for the prevention and treatment of diabetes mellitus.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA

Projekti:
022-0222148-2125 - Mutageni i antimutageni u ekogenetičkim istraživanjima (Garaj-Vrhovac, Vera, MZOS ) ( CroRIS)
119-0000000-1255 - Kemoprevencija rasta tumora polifenolnim sastavnicama (Oršolić, Nada, MZOS ) ( CroRIS)
119-0532265-1254 - Imunoadjuvantno i zaštitno djelovanje propolisa u životinja (Benković, Vesna, MZOS ) ( CroRIS)

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb

Profili:

Ivan Kosalec (autor)