Naslov
Alternations in number of calcium-binding striatal and hippocampal parvalbumin and calbindin D28k interneurons in the brain of St3Gal2, St3Gal3 and double knockout mice

Autori
Blažetić, Senka ; Viljetić, Barbara ; Labak Irena ; Balog Marta ; Ivić Vedrana ; Schnaar Ronald L. ; Heffer Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts ; 4.Croatian Congres of Neuroscience / Croatian Society for Neuroscience (CSfN) - Zagreb, 2013, 44-45

Skup
4.Croatian Congres of Neuroscience

Mjesto i datum
Zagreb, Hrvatska, 20.09.2013. - 21.09.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
parvalbumin; calbindin; interneurons; hippocampus; striatum; mouse

Sažetak
The striatum is a central component of the basal ganglia that collects and processes information coming from the cerebral cortex and the thalamus. In the striatum, more than 95% of neurons are GABAergic medium-sized spiny neurons (MSNs), which form two intermingled populations distinguished by their projections and protein content. Also, striatum is a key neural substrate for procedural learning and memory. The hippocampal formation has a central role in spatial navigation. This study investigated two groups of calcium-binding GABAergic non-pyramidal cells: parvalbumin (PV) fast-skipping (FS) cells with morphology of basket or chandelier neurons and calbindin D28k (CB) positive cells marked as non fast-skipping (NFS) expressing cells in brain of St3Gal2 (beta- galactoside alpha-2, 3-sialyltransferase 2) null, St3Gal3 (ST3 beta-galactoside alpha-2, 3- sialyltransferase 3) null and double null (DKO) mice. Free floating immunohistochemical method was used to determine the density and distribution of interneurons in coronal sections of mice brain. Quantification of neurons was made by using ImageJ processing and analysis application. PV and CB neurons were quantified relative to all neurons (identified by NeuN). Our results showed that there is a significant difference in number of calcium-binding interneurons of knock-out mice in striatum and hippocampus. ST3Gal2 mice had a significantly lower proportion of CB positive neurons in striatum and hippocampus. In hippocampus of ST3Gal2 mice there is also lower proportion of PV interneurons and there is no PV interneurons in granule cell layer of the dentate gyrus in comparation with wild tipe and other knock-out mice. These findings are the first to report about PV and CB interneurons in the striatum and hippocampus of these mice and may help to illuminate the role of sialic acid epitopes in the production and migration of striatal interneurons.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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