Naslov
Epigenetic characterization of chromatin in cycling cells of pedunculate oak, Quercus robur L.

Autori
Vičić, Vedrana ; Barišić, Darko ; Horvat, Tomislav ; Biruš, Ivan ; Zoldoš, Vlatka

Izvornik
Tree genetics & genomes (1614-2942) 9 (2013); 1247-1256

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
interphase chromatin; chromocenters; DNA methylation; epigenetics; histone acetylation; histone methylation; Quercus robur

Sažetak
Cycling cells of Quercus robur have a simple nuclear organization where most of the heterochromatin is visible as DAPI-positive chromocenters, which correspond to DAPI bands at the (peri)centromeric region of each of the 24 chromosomes of the oak complement. Immunofluorescence using 5-mC revealed dispersed distribution of the signal throughout the interphase nucleus/chromosomes without enrichment within DAPI-positive chromocenters/bands, suggesting that DNA methylation was not restricted to constitutive heterochromatin, but was associated with both euchromatic and heterochromatic domains. While H3K9ac exhibited typical euchromatin-specific distribution, the distributional pattern of histone methylation marks H3K9me1, H3K27me2, and H3K4me3 showed some specificity. The H3K9me1 and H3K27me2, both heterochromatin-associated marks, were not restricted to chromocenters, but showed additional dispersed distribution within euchromatin, while H3K27me2 mark also clustered in foci that did not co-localize with chromocenters. Surprisingly, even though H3K4me3 was distributed in the entire chromatin, many chromocenters were enriched with this euchromatin-specific modification. We discuss the distribution of the epigenetic marks in the context of the genome composition and lifestyle of Q. robur.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA