Pregled bibliografske jedinice broj: 643112
Structural analysis of brain ganglioside acetylation patterns in mice with altered ganglioside biosynthesis
Structural analysis of brain ganglioside acetylation patterns in mice with altered ganglioside biosynthesis // Carbohydrate research, 382 (2013), 1-8 doi:10.1016/j.carres.2013.09.007 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 643112 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Structural analysis of brain ganglioside acetylation patterns in mice with altered ganglioside biosynthesis
Autori
Mlinac, Kristina ; Fabris, Dragana ; Vukelić, Željka ; Rožman, Marko ; Heffer, Marija ; Kalanj Bognar, Svjetlana
Izvornik
Carbohydrate research (0008-6215) 382
(2013);
1-8
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
brain gangliosides; mass spectrometric characterization; O-acetylated ganglioside species; O-acetylation patterns; St8sia1 null and B4galnt1 null mice
Sažetak
Gangliosides are sialylated membrane glycosphingolipids especially abundant in mammalian brain tissue. Sialic acid O-acetylation is one of the most common structural modifications of gangliosides which considerably influences their chemical properties. In this study, gangliosides extracted from brain tissue of mice with altered ganglioside biosynthesis (St8sia1 null and B4galnt1 null mice) were structurally characterized and their acetylation pattern was analyzed. Extracted native and alkali-treated gangliosides were resolved by high performance thin layer chromatography. Ganglioside mixtures as well as separated individual ganglioside fractions were further analyzed by tandem mass spectrometry. Several O-acetylated brain ganglioside species were found in knockout mice, not present in the wild-type mice. To the best of our knowledge this is the first report on the presence of O-acetylated GD1a in St8sia1 null mice and O-acetylated GM3 species in B4galnt1 null mice. In addition, much higher diversity of abnormally accumulated brain ganglioside species regarding the structure of ceramide portion was observed in knockout versus wild-type mice. Obtained findings indicate that the diversity of brain ganglioside structures as well as acetylation patterns in mice with altered ganglioside biosynthesis, is even higher than previously reported. Further investigation is needed in order to explore the effects of acetylation on ganglioside interactions with other molecules and consequently the physiological role of acetylated ganglioside species.
Izvorni jezik
Engleski
Znanstvena područja
Kemija
POVEZANOST RADA
Projekti:
098-0982915-2945 - Spektroskopija, kemijska svojstva i reakcije biološki aktivnih molekula (Kovač, Branka, MZOS ) ( CroRIS)
108-1081870-1877 - Uloga membranskih lipida u moždanom razvitku, starenju i neurodegeneraciji (Kalanj-Bognar, Svjetlana, MZOS ) ( CroRIS)
108-1081870-2415 - Strukturno-funkcionalna glikolipidomika moždanog razvitka i maligne alteracije (Vukelić, Željka, MZOS ) ( CroRIS)
219-0061194-2158 - Uloga lipidnih splavi i glikokonjugata u razvoju i regeneraciji živčanog sustava (Heffer, Marija, MZOS ) ( CroRIS)
Ustanove:
Institut "Ruđer Bošković", Zagreb,
Medicinski fakultet, Zagreb,
Medicinski fakultet, Osijek
Profili:
Kristina Mlinac Jerković
(autor)
Marko Rožman
(autor)
Svjetlana Kalanj-Bognar
(autor)
Marija Heffer
(autor)
Željka Vukelić
(autor)
Dragana Fabris
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
Uključenost u ostale bibliografske baze podataka::
- AGRICOLA
- BIOSIS Previews (Biological Abstracts)
- EMBASE (Excerpta Medica)
- MEDLINE