Naslov
Effect of variable selenium and zinc food content on the level of oxidative stress in Sprague-Dawley rats

Autori
Ćosić, Anita ; Novak, Sanja ; Čavka, Ana ; Popović, Brigita ; Lončarić, Zdenko ; Drenjančević, Ines

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Periodicum Biologorum, Vol 115, Suppl 2 / Vitale, Branko ; Drenjancevic, Ines - Zagreb, 2013, 21---

Skup
3. Congress of Croatian Physiological Society and 1. Regional Congress of The Physiologycal Societies

Mjesto i datum
Rijeka, Hrvatska, 13.09.2013. - 15.09.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
oxidative stress ; zinc ; selenium

Sažetak
AIM: The aim of this study was to investigate whether food enriched with selenium (Se) and/or zinc (Zn)can effect serum oxidative stress in male rats. METHODS: 4-weeks old male Sprague- Dawley were divided inti 3 groups:control group fed with low- Se/low-Zn wheat ; rats fed with high Se/low- Zn wheat ; and group fed with high-Zn/low-Se. After 10 weeks rats were sacrified for serum collection followed by measurement of Ferric reducing ability of plasma (FRAP), Thiobarbituric Acid Reactive Substances (TBARS ; reaction of malonilaldehyde (MDA) with thiobarbituric acid) and serum glutation peroxidase 1 (GPX1)). RESULTS: 10 weeks of feeding with food enriched with Se and Zn did not cause any significant changes in FRAP or TBARS. nevertheless, GPX1 serum level was significantly lower in high-Zn/low-Se rats compared to controls and high-Zn/low-Se rats (P<0, 001). there was no dofference between control and high-Se/low-Zn rats. CONCLUSION: The results of this study showed that serum antioxidative capacity has not been affected by changesin Se/Zn food content, however, the level GPX1 was significantly increased in rats fed with Se enriched food, suggesting that changes in Se intake can affect GPX1 levels in the blood.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA