Naslov
The assessment of DNA damage in poultry spermatozoa after exposure to low doses of ionising radiation

Autori
Milić, Mirta ; Vilić, Marinko ; Kašuba Vilena, Pejaković Hlede, Jadranka ; Gottstein Željko ; Karadjole, Martina ; Miljanić Saveta

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), ostalo

Izvornik
Zbornik radova 9. simpozija hrvaskog društva za zaštitu od zračenja / Knežević, Željka ; Majer Marija ; Bronić Krajcar, Ines - Zagreb : Hrvatsko društvo za zaštitu od zračenja, 2013, 147-151

ISBN
978-953-96133-8-7

Skup
9. SIMPOZIJ HRVATSKOG DRUŠTVA ZA ZAŠTITU OD ZRAČENJA

Mjesto i datum
Krk, Hrvatska, 10.04.2013. - 12.04.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Spermatozoa; Ionising radiation; DNA damage

Sažetak
The assessment of DNA damage in poultry spermatozoa after exposure to low doses of ionising radiation using comet assay (alkaline and neutral) was not previously reported. The aim of the study was to investigate the effects of different doses of gamma-radiation on tail length (TL), tail intensity (TI) and tail moment (TM) of cocks’ spermatozoa using comet assay. Semen samples of 5 cocks of heavy line (45 weeks old) were collected by dorso-abdominal massage method. All samples were diluted and irradiated in vitro with doses of 0.3, 0.5, 1 and 2 Gy. One-way ANOVA was used to compare differences in DNA damage. Results showed high sensitivity of the method even at low dose exposure from 0.3 Gy, with no dose related relationship, but with increase of apoptotic cells. Damage distribution was spread more after 0.5 Gy, 1 Gy and 2 Gy dose. TI and TM were lower than in fresh semen cock samples (other researchers), while TL was little bit higher. Lower damage for higher doses 0.3 Gy was strange result, and since semen samples do not have DNA repair mechanisms, further larger studies are needed to answer that question.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

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