Naslov
Mapping the polypeptide chain organization of the main extracellular domain of the alpha-subunit in membrane-bound acetylcholine receptor by antipeptide antibodies spanning the entire domain

Autori
Atassi, M. Zouhair ; Mulac-Jeričević Biserka ; Ashizawa, Tetsuo

Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, ostalo

Knjiga
Mapping the polypeptide chain organization of the main extracellular domain of the alpha-subunit in membrane-bound acetylcholine receptor by antipeptide antibodies spanning the entire domain

Urednik/ci
Atassi, M. Zouhair

Izdavač
Plenum Press

Grad
New York (NY)

Godina
1994

Raspon stranica
221-228

ISBN
0306446650

Ključne riječi
acetylcholine receptor

Sažetak
To study the organization of the polypeptide chain of the main extracellular domain of the nicotinic acetylcholine receptor (AChR) alpha-subunit, we examined the ability of the native membrane-bound AChR of Torpedo californica (T-AChR) to bind a panel of antibodies against overlapping synthetic peptides which collectively encompassed this entire domain. Antibodies against the alpha-chain peptides alpha 1-16, alpha 89-104 and alpha 158-174 were able to bind to membrane-bound T-AChR. Other anti-peptide antibodies showed little or no binding to T-AChR in the membrane. It is concluded that regions alpha 1-16, alpha 89-104 and alpha 158-174 are highly exposed on the surface of the alpha subunit of membrane-bound AChR.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA