Naslov
Urinary analysis reveals high deoxynivalenol exposure in pregnant women from Croatia

Autori
Šarkanj, Bojan ; Warth, Benedikt ; Uhlig, Silvio ; Abiab, Wilfred A. ; Sulyok, Michael ; Klapec, Tomislav ; Krska, Rudolf ; Banjari, Ines

Izvornik
Food and chemical toxicology (0278-6915) 62 (2013); 231-237

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
mycotoxins; deoxynivalenol glucuronide; biomarker; pregnant women; human urine; Croatia

Sažetak
In this pilot survey the levels of various mycotoxin biomarkers were determined in third trimester pregnant women from eastern Croatia. First void urine samples were collected and analysed using a “dilute and shoot” LC-ESI-MS/MS multi biomarker method. Deoxynivalenol (DON) and its metabolites: deoxynivalenol-15-glucuronide and deoxynivalenol-3-glucuronide were detected in 97.5% of the studied samples, partly at exceptionally high levels, while ochratoxin A was found in 10% of the samples. DON exposure was primarily reflected by the presence of deoxynivalenol-15-glucuronide with a mean concentration of 120 μg L-1, while free DON was detected with a mean concentration of 18.3 μg L-1. Several highly contaminated urine samples contained a third DON conjugate, tentatively identified as deoxynivalenol-7-glucuronide by MS/MS scans. The levels of urinary DON and its metabolites measured in this study are the highest ever reported, and 48% of subjects were estimated to exceed the provisional maximum tolerable daily intake (1 μg kg-1 b.w.).

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

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