Naslov
Effect of bred on efficiency of oocyte collection and subsequent bovine embryo production

Autori
Lojkić, Martina ; Getz, Iva ; Samardžija, Marko ; Maćešić, Nino ; Matković, Mario ; Karadjole, Tugomir ; Bačić, Goran ; Prvanović Babić, Nikica ; Grizelj, Juraj ; Vince, Silvijo ; Folnožić, Ivan

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Congress Proceedings XIII Middle European Buiatrics Congress / Gvozdić, Dragan ; Petrujkić, Branko - Beograd : Serbian Buiatrics Association, Faculty of Veterinary Medicine, 2013, 426-431

ISBN
978-86-916767-0-4

Skup
XIII Middle European Buiatrics Congress

Mjesto i datum
Beograd, Srbija, 05.06.2013. - 08.06.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
bovine; follicle aspiration; breed; IVF; embryo development

Sažetak
The aim of the present study was to compare the efficiency Ovum Pick-Up (OPU) and subsequent blastocyst development of oocytes collected from three cattle breeds. OPU was performed in 14 nonlactating donor cows (4 Simmental (S), 5 Charolais (CH) and 5 Holstein-Friesian (HF)). Cows were synchronized with PGF2 and stimulated with pFSH, twice a day during two days (Folltropin®, Bioniche, a total dose: 200 mg NIH-FSH-P1). Four OPU sessions were performed per cow. The number of aspirated follicles, the number of retrieved oocytes and oocyte recovery rate were recorded. The recovered cumulus oocyte complexes (COCs) were morphologically classified into four (1-4) quality categories. Grade 1 and 2 oocytes were matured, fertilized and cultured in vitro for 9 days. Embryo yield was recorded at day 5, 6, 7 and 9. The number of retrieved COCs per OPU session was significantly higher for Simmental and Charolais donor cows comparing to Holstein-Friesian cows (9, 5, 8, 9 and 4, 6 respectively), due to a higher number of follicles present in the Simmental and Charolais ovaries. In terms of blastocyst rates at days 5, 6, 7 and 9 of in vitro culture, no statistical differences were observed between breeds. In conclusion, we reported that Simmental and Charolais donor cows showed significantly better results in number of follicles punctured, total number of oocytes and number of grade 1 and 2 oocytes recovered by OPU, but there were no differences in developmental competence of retrieved oocytes of similar quality between three donor breeds.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina, Biotehnologija

POVEZANOST RADA