Naslov
DADP: the Database of Anuran Defense Peptides

Autori
Novković, Mario ; Simunić, Juraj ; Bojović, Viktor ; Tossi, Alessandro ; Juretić, Davor

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
New Antimicrobials Workshop in Trieste, 25-26.5.2012 / Tossi, Alessandro - Trst : Edizioni Università di Trieste, 2012, 39-40

ISBN
978-88-8303-379-7

Skup
New Antimicrobials Project 2-nd Workshop. New Compounds & New Strategies for Antimicrobials

Mjesto i datum
Trst, Italija, 25.05.2012. - 26.05.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
anuran; host defense; peptides; antibiotics; signal peptide; database

Sažetak
Anuran tissues, and especially skin, are a rich source of bioactive peptides and their precursors. As potential antibiotics, host defense peptides from amphibian species are a fast growing group in the UniProtKB database. We here present a manually curated database (http://split4.pmfst.hr/dadp/) of antimicrobial and other defense peptides with a total of 2571 entries, 1766 full precursors with demarcated signal peptide (SP), acidic proregion(s) and bioactive moiety(s) and 805 bioactive defense peptides, encompassing a total of 167 anuran species from 12 families [1]. Each DADP entry ID contains detailed information about peptide names and sequences, tripartite structure, taxonomy, biogeography data (ecozone and distribution), SP class, tissue, biological function, links to UniProtKB, references and activity data in the form of minimal inhibitory concentration (MIC, µM) for E. coli and/or S. aureus and concentration required for 50% haemolysis of red blood cells (HC50). Precursor structure is informative about origin, function and evolution of anuran host defense peptides, while more than 900 mature peptides with known MIC values in DADP are a valuable resource for structure-activity studies. The DADP focus on precursors and their conserved SPs has allowed the individuation of six distinct SP classes. The more conserved of these can be used for searching cDNA, UniProtKB or other protein databases for novel bioactive peptides, for creating PROSITE search patterns, and for phylogenetic analysis. [1] M. Novković, J. Simunić, V. Bojović, A. Tossi and D. Juretić, DADP: the Database of Anuran Defense Peptides, Bioinformatics J., (in press 2012).

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Kemija, Biologija

POVEZANOST RADA