Pregled bibliografske jedinice broj: 636534
QRT-PCR analysis of HMGA2 expression in FNA samples of thyroid papillary carcinoma and non-neoplastic thyroid lesions
QRT-PCR analysis of HMGA2 expression in FNA samples of thyroid papillary carcinoma and non-neoplastic thyroid lesions // Acta Cytologica, vol. 57, suppl. 1
Pariz, Francuska, 2013. str. 32-32 (predavanje, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 636534 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
QRT-PCR analysis of HMGA2 expression in FNA samples of thyroid papillary carcinoma and non-neoplastic thyroid lesions
Autori
Mateša, Neven ; Šamija, Ivan ; Lukač, Josip ; Kusić, Zvonko
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Acta Cytologica, vol. 57, suppl. 1
/ - , 2013, 32-32
Skup
18th International Congress of Cytology
Mjesto i datum
Pariz, Francuska, 26.05.2013. - 30.05.2013
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
cytology; HMGA2; RT-PCR; thyroid
Sažetak
Objectives: To determine and compare HMGA2 expression in non-neoplastic thyroid lesions and thyroid papillary carcinoma in fine needle aspiration (FNA) samples by quantitative real-time RT-PCR (QRTPCR). Materials and Methods: QRT-PCR for HMGA2 expression was performed on RNA isolated from aspirates obtained by ultrasound guided FNA from 95 patients with papillary carcinoma, Hashimoto thyroiditis and nodular goitre. HMGA2 expression was quantified by DDCt method using pooled FNA samples from normal thyroid tissue as calibrator. Results of QRT-PCR analysis were evaluated against the definitive FNA diagnosis. Results:With a cut-off value of 11.69 determined by ROC curve analysis 18 out of 20 (90%) papillary carcinoma, 2 out of 28 (7%) Hashimoto thyroiditis and 6 out of 47 (13%) nodular goitre samples were positive for HMGA2 expression. HMGA2 expression has shown accuracy of 89.4%, sensitivity of 90.0% and specificity of 90.1% in discriminating between samples with papillary carcinoma and non-neoplastic thyroid lesions. Proportion of HMGA2 positive samples was significantly higher in samples with papillary carcinoma compared to samples with non-neoplastic lesions (p<0.0001, Fisher’s exact test). There was no significant difference in the proportion of positive samples between Hashimoto thyroiditis and nodular goitre samples. Conclusion: Analysis of HMGA2 expression by QRT-PCR could improve conventional thyroid cytology in diagnosis of papillary carcinoma
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti
POVEZANOST RADA
Projekti:
134-1342428-2430 - Karcinogeneza u štitnjači i gušavost u Hrvatskoj (Kusić, Zvonko, MZOS ) ( CroRIS)
Ustanove:
KBC "Sestre Milosrdnice"
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Emerging Sources Citation Index (ESCI)
- Scopus
- MEDLINE