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Pregled bibliografske jedinice broj: 636534

QRT-PCR analysis of HMGA2 expression in FNA samples of thyroid papillary carcinoma and non-neoplastic thyroid lesions


Mateša, Neven; Šamija, Ivan; Lukač, Josip; Kusić, Zvonko
QRT-PCR analysis of HMGA2 expression in FNA samples of thyroid papillary carcinoma and non-neoplastic thyroid lesions // Acta Cytologica, vol. 57, suppl. 1
Pariz, Francuska, 2013. str. 32-32 (predavanje, međunarodna recenzija, sažetak, znanstveni)


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Naslov
QRT-PCR analysis of HMGA2 expression in FNA samples of thyroid papillary carcinoma and non-neoplastic thyroid lesions

Autori
Mateša, Neven ; Šamija, Ivan ; Lukač, Josip ; Kusić, Zvonko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Acta Cytologica, vol. 57, suppl. 1 / - , 2013, 32-32

Skup
18th International Congress of Cytology

Mjesto i datum
Pariz, Francuska, 26.05.2013. - 30.05.2013

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
cytology; HMGA2; RT-PCR; thyroid

Sažetak
Objectives: To determine and compare HMGA2 expression in non-neoplastic thyroid lesions and thyroid papillary carcinoma in fine needle aspiration (FNA) samples by quantitative real-time RT-PCR (QRTPCR). Materials and Methods: QRT-PCR for HMGA2 expression was performed on RNA isolated from aspirates obtained by ultrasound guided FNA from 95 patients with papillary carcinoma, Hashimoto thyroiditis and nodular goitre. HMGA2 expression was quantified by DDCt method using pooled FNA samples from normal thyroid tissue as calibrator. Results of QRT-PCR analysis were evaluated against the definitive FNA diagnosis. Results:With a cut-off value of 11.69 determined by ROC curve analysis 18 out of 20 (90%) papillary carcinoma, 2 out of 28 (7%) Hashimoto thyroiditis and 6 out of 47 (13%) nodular goitre samples were positive for HMGA2 expression. HMGA2 expression has shown accuracy of 89.4%, sensitivity of 90.0% and specificity of 90.1% in discriminating between samples with papillary carcinoma and non-neoplastic thyroid lesions. Proportion of HMGA2 positive samples was significantly higher in samples with papillary carcinoma compared to samples with non-neoplastic lesions (p<0.0001, Fisher’s exact test). There was no significant difference in the proportion of positive samples between Hashimoto thyroiditis and nodular goitre samples. Conclusion: Analysis of HMGA2 expression by QRT-PCR could improve conventional thyroid cytology in diagnosis of papillary carcinoma

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti



POVEZANOST RADA


Projekti:
134-1342428-2430 - Karcinogeneza u štitnjači i gušavost u Hrvatskoj (Kusić, Zvonko, MZOS ) ( CroRIS)

Ustanove:
KBC "Sestre Milosrdnice"

Profili:

Avatar Url Neven Mateša (autor)

Avatar Url Ivan Šamija (autor)

Avatar Url Zvonko Kusić (autor)

Avatar Url Josip Lukač (autor)


Citiraj ovu publikaciju:

Mateša, Neven; Šamija, Ivan; Lukač, Josip; Kusić, Zvonko
QRT-PCR analysis of HMGA2 expression in FNA samples of thyroid papillary carcinoma and non-neoplastic thyroid lesions // Acta Cytologica, vol. 57, suppl. 1
Pariz, Francuska, 2013. str. 32-32 (predavanje, međunarodna recenzija, sažetak, znanstveni)
Mateša, N., Šamija, I., Lukač, J. & Kusić, Z. (2013) QRT-PCR analysis of HMGA2 expression in FNA samples of thyroid papillary carcinoma and non-neoplastic thyroid lesions. U: Acta Cytologica, vol. 57, suppl. 1.
@article{article, author = {Mate\v{s}a, Neven and \v{S}amija, Ivan and Luka\v{c}, Josip and Kusi\'{c}, Zvonko}, year = {2013}, pages = {32-32}, keywords = {cytology, HMGA2, RT-PCR, thyroid}, title = {QRT-PCR analysis of HMGA2 expression in FNA samples of thyroid papillary carcinoma and non-neoplastic thyroid lesions}, keyword = {cytology, HMGA2, RT-PCR, thyroid}, publisherplace = {Pariz, Francuska} }
@article{article, author = {Mate\v{s}a, Neven and \v{S}amija, Ivan and Luka\v{c}, Josip and Kusi\'{c}, Zvonko}, year = {2013}, pages = {32-32}, keywords = {cytology, HMGA2, RT-PCR, thyroid}, title = {QRT-PCR analysis of HMGA2 expression in FNA samples of thyroid papillary carcinoma and non-neoplastic thyroid lesions}, keyword = {cytology, HMGA2, RT-PCR, thyroid}, publisherplace = {Pariz, Francuska} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
    • Emerging Sources Citation Index (ESCI)
  • Scopus
  • MEDLINE





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