Naslov
Expression of hepatic and renal sulfate anion transporter SAT1 (SLC26A1) in rats treated with ethylene glycol

Autori
Brzica, Hrvoje ; Breljak, Davorka ; Vrhovac, Ivana ; Micek, Vedran ; Lovrić, Mila ; Burckhardt, Gerhard ; Burckhardt, Birgitta C ; Sabolić, Ivan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Arhiv za higijenu rada i toksikologiju 64(2) / Kopjar, Nevenka - Zagreb : Institut za medicinska istraživanja i medicinu rada, 2013, 341-341

Skup
1. hrvatski simpozij o transporterima SOT-1

Mjesto i datum
Zagreb, Hrvatska, 06.06.2013. - 07.06.2013

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
gender differences; immunocytochemistry; kidney; liver; nephrolithiasis; oxalemia; oxaluria; real time RT-PCR; urolitiasis; Western blotting

Sažetak
Oxalate (Ox) urolithiasis is a systemic disorder that affects ~10 % of the adult human population with a male prevalence. It occurs ~2.5 times more often in men than in women. A trigger for urolithiasis is urine oversaturation with a crystal-forming material such as CaOx, where Ox originates from the liver metabolism and/or absorption from food. The Slc26 family of multifunctional anion exchangers, among which Sat-1 (Slc26a1) and chloride/formate exchanger CFEX (Slc26a6) are prime examples, is responsible for Ox trafficking in the organism. The aim of this work was to test the role of hepatic and renal Sat-1 in the generation of Ox urolithiasis in a rat model of this disease, following treatment with ethylene glycol (EG). Three-month-old male (M) and female (F) Wistar rats were divided into control (drank tap water) and EG-treated (drank 0.75 % EG in tap water for one month) groups. Urine and blood serums were sampled and tested for Ox crystals and concentration, while liver and kidney tissue samples were used in morphological, immunocytochemical, Western-blotting, and real-time RT-PCR studies. In EG-treated animals, M but not F exhibited hyperoxalemia, hyperoxaluria and large Ox crystals in their urine. However, the expression of Sat-1 protein was upregulated in both the liver and kidneys of EG treated F but not M. The expression of Sat-1 mRNA remained unchanged in all of the groups, indicating an involvement of a post- transcriptional regulation of protein expression. We conclude that Sat-1 does not contribute significantly to the generation of Ox urolithiasis in rats.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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