Naslov
Importance of ABC transporters in earthworm Eisenia fetida can be compromised by environmental influences
(Importance of ABC transporters in earthworm eisenia fetida can be compromised by environmental influences)

Autori
Babić, Sanja ; Bošnjak, Ivana ; Bielen, Ana ; Likić, Saša ; Šver, Lidija ; Topić Popović, Natalija ; Strunjak-Perović, Ivančica ; Barišić, Josip ; Čož-Rakovac, Rozelinda ; Sauerborn Klobučar, Roberta

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
The 1st Croatian Symposium on Membrane Transporters : abstracts = 1. hrvatski simpozij o transporterima (SOT-1) : knjiga sažetaka ; u: Arhiv za higijenu rada i toksikologiju 64(2013)(2) 331-341 / Kopjar, Nevenka - Zagreb : Institut za medicinska istraživanja i medicinu rada, 2013, 341-341

Skup
Hrvatski simpozij o transporterima (1 ; 2013)

Mjesto i datum
Zagreb, Hrvatska, 06.06.2013. - 07.06.2013

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
bisphenol A; effl ux transporters; modulators; multixenobiotic resistance (MXR); P-glycoprotein; snow samples

Sažetak
In many organisms, the multixenobiotic resistance mechanism (MXR) is mediated by the activity of ABC transporters that bind and actively effl ux different toxic substrates from cells. This study attempted to identify MXR-related genes in the earthworm Eisenia fetida and compare the impact of the selected modulators, including verapamil, cyclosporine A, MK571, probenecid, orthovanadate, bisphenol A, and snow samples on the earthworm’s MXR. To confi rm the presence of MXR-reversing agents in E. fetida, we measured the accumulation of model substrates rhodamine B and rhodamine 123 in the body tissue of an adult earthworm. Experiments performed using the fi lter paper contact test showed that all of the tested modulators and environmental samples signifi cantly inhibited transport activity of the model substrates. Furthermore, partial mRNA sequence coding for P-glycoprotein (P-gp/Abcb1) was identifi ed and showed a high amino acid identity (up to 78 %) with homologues from different organisms. We could say that the ABC transporters are involved in various specifi c mechanisms that help earthworms to survive in a polluted environment and that their effi ciency can become compromised by environmental contamination.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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