Naslov
GSTM1 AND GSTT1 GENETIC POLYMORPHISM, AND ANTHROPOLOGICAL AND OXIDATIVE STRESS BIOMARKERS IN SUBJECTS WITH PTSD - A PILOT STUDY

Autori
Delaš, Marija ; Aždajić, Stjepan ; Borovac-Štefanović, Leda ; Kalinić, Dubravka ; Beer-Ljubić, Blanka ; Delaš, Ivančica ; Pašalić, Daria

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Biochimia Clinica / Panteghini, Mauro - Milano, 2013

Skup
EuroMedLab Milan 2013

Mjesto i datum
Milano, Italija, 19.05.2013. - 23.05.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
PTSD; GSTT1; GSTM1; genetic polymorphism

Sažetak
Background. Posttraumatic stress disorder (PTSD) is a complex anxiety disorder caused by a traumatic experience. It is over represented in combat veterans from Croatian homeland war. The glutathione S-transferases (GSTs) belong to a group of major detoxifying enzymes responsible for eliminating of oxidative stress products. It is hypothesized that GSTs genetic isoforms together with oxidative stress biomarkers might contribute in pathogenesis of PTSD. Methods. 134 male combat veterans from Croatian homeland war were included in the study, 121 with PTSD diagnosed according to Diagnostic and Statistical Manual of Mental Disorders (DSM-IV), and 13 control subjects. Concentrations of serum lipid parameters were determined with standard enzymatic methods. Gluthatione peroxidase (GPx) and superoxide dismutase (SOD) catalytic concentrations were determined in hemolysates of erythrocytes by standard spectrophotometric methods. Anthropometric data were collected. GSTM1 and GSTT1 were determined by multiplex PCR. Results. GSTM1 genotype frequencies (functional vs. deleted) were 95 vs. 26 in PTSD, and 9 vs. 4 in controls, respectively, (P=0.487). GSTT1 genotype frequencies (functional vs. deleted) were 97 vs. 22 in PTSD, and 9 vs.4 in controls, respectively, (P=0.487). Waist-to-hip ratio (WHR), SOD and GPx were significantly lower in PTSD subjects (P=0.040, P <0.001 and P=0.001, respectively). There was a significant difference in WHR values between GSTM1 genotype subgroups in subjects with PTSD (P=0.001). WHR-median in subjects with at least one GTSM1 functional allele was 0.94 (25th and 75th percentiles were 0.89 and 0.97), while in subjects with both deleted alleles WHR median was 1.01 (25th and 75th percentiles were 0.89 and 1.03). Spearman rank correlation test showed significant correlations in subjects with PTSD for WHR values and GSTM1 genotypes (rho=-0.597, p <0.001), SOD and GPx (rho=0.503, P <0.001) and low correlation between GPx and MDA (rho=-0.274, P=0.054). Conclusions. The results indicate that GSTs genetic polymorphism might be associated with development of PTSD by altering the values of oxidative stress markers. It is evidenced that different genetic, anthropometric and biochemical markers might be involved in pathogenesis of PTSD

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA