Naslov
INFLUENCE OF UVC RADIATION ON BLOOD, SALIVA AND SEMEN SAMPLES

Autori
Gršković, Branka ; Zrnec, Dario ; Popović, Maja ; Petek, Maja Jelena ; Primorac, Dragan ; Mršić, Gordan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
PROGRAM AND ABSTRACTS THE EIGHTH ISABS CONFERENCE IN FORENSIC, ANTHROPOLOGIC AND MEDICAL GENETICS AND MAYO CLINIC LECTURES IN TRANSLATIONAL MEDICINE / Stanimir Vuk-Pavlović, Dragan Primorac, Moses Schanfield - Split : International Society for Applied Biological Sciences (ISABS), 2013, 161-161

ISBN
978-953-57695-0-7

Skup
The Eighth ISABS Conference in Forensic, Anthropologic and Medical Genetics and Mayo Clinic Lectures in Translational Medicine

Mjesto i datum
Split, Hrvatska, 24.06.2013. - 28.06.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
ultraviolet C (UVC) radiation; blood; saliva; semen; DNA; contamination of forensic evidence

Sažetak
The major threat in forensic laboratories is a sample mix-up and contamination of forensic evidence with foreign DNA, which could lead to identification of mixed DNA profiles, inhibition of PCR reactions or wrong DNA genotyping. The aim was to study in real conditions the true possibility of working surfaces contamination in the laboratory and the anti-contamination effects of commercial cleaning agents and ultraviolet C (UVC) radiation on naked DNA, diluted and non-diluted blood, semen, saliva samples and controls. The doses of UVC radiation, which would cause a complete loss of DNA profile in biological samples were also investigated. Buccal swab sample, blood, saliva and semen were obtained from a single male donor. Genomic DNA was extracted using Chelex. DNA was amplified using the AmpFlSTR® NGM™ PCR Amplification Kit. Statistical data analysis was performed using Excel. DNA concentration, UVC dose, sample volume, radiation time, number of correctly detected alleles on genetic loci and number of correctly detected alleles in four loci groups were tested. To our knowledge, the rate of UVC induced DNA damage of semen stains was not analyzed so far. The results of this study pointed out the inefficiency of commercial cleaning agents for working surfaces decontamination, when dealing with non-diluted blood and semen. High UVC doses are necessary for complete decontamination of working surfaces with inflicted non-diluted blood and semen controls. It is recommended to carefully and thoroughly clean working surfaces with commercial agents followed by minimal 16 hour UVC exposure (dose approximately 4380 mJ/cm2) for complete and successful decontamination.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

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