Naslov
A comprehensive analysis of varicella zoster virus proteins using a new monoclonal antibody collection

Autori
Lenac Roviš, Tihana ; Bailer, S.M. ; Pothineni, V.R. ; Ouwendijk, W.J. ; Šimić, Hrvoje ; Babić, Marina ; Miklić, Karmela ; Malić, Suzanna ; Verweij, M.C. ; Baiker, A. ; Gonzalez, O. ; von Brunn, A. ; Zimmer, R. ; Früh, K. ; Verjans, G.M. ; Jonjić, Stipan ; Haas, J.

Izvornik
Journal of virology (0022-538X) 87 (2013), 12; 6943-6954

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Varicella-zoster virus; monoclonal antibody; simian varicella virus

Sažetak
Varicella-zoster virus (VZV) is the etiological agent of chickenpox and shingles. Due to the virus’s restricted host and cell type tropism and the lack of tools for VZV proteomics, it is one of the least-characterized human herpesviruses. We generated 251 monoclonal antibodies (MAbs) against 59 of the 71 (83%) currently known unique VZV proteins to characterize VZV protein expression in vitro and in situ. Using this new set of MAbs, 44 viral proteins were detected by Western blotting (WB) and indirect immunofluorescence (IF) ; 13 were detected by WB only, and 2 were detected by IF only. A large proportion of viral proteins was analyzed for the first time in the context of virus infection. Our study revealed the subcellular localization of 46 proteins, 14 of which were analyzed in detail by confocal microscopy. Seven viral proteins were analyzed in time course experiments and showed a cascade-like temporal gene expression pattern similar to those of other herpesviruses. Furthermore, selected MAbs tested positive on human skin lesions by using immunohistochemistry, demonstrating the wide applicability of the MAb collection. Finally, a significant portion of the VZV-specific antibodies reacted with orthologs of simian varicella virus (SVV), thus enabling the systematic analysis of varicella in a nonhuman primate model system. In summary, this study provides insight into the potential function of numerous VZV proteins and novel tools to systematically study VZV and SVV pathogenesis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA