Pregled bibliografske jedinice broj: 629287
Database and data analysis application for ganglioside characterization by mass spectrometry and tandem mass spectrometry
Database and data analysis application for ganglioside characterization by mass spectrometry and tandem mass spectrometry // 31st Informal Meeting on Mass Spectrometry, Palermo, Italia
Palermo, 2013. (predavanje, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 629287 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Database and data analysis application for ganglioside characterization by mass spectrometry and tandem mass spectrometry
Autori
Rožman, Marko ; Fabris, Dragana ; Mrla, Tomislav ; Vukelić, Željka
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
31st Informal Meeting on Mass Spectrometry, Palermo, Italia
/ - Palermo, 2013
Skup
31st Informal Meeting on Mass Spectrometry
Mjesto i datum
Palermo, Italija, 05.05.2013. - 08.05.2013
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
glycosphingolipid identification; database; tandem mass spectrometry
Sažetak
Background: Gangliosides as important building blocks of animal cell membranes serve as determinants in cellular recognition and cell-to-cell communication but also reflect abnormalities in cell architecture due to different pathophysiological events. Objective: In order to enable higher throughput and to facilitate the characterization of gangliosides by MS and tandem MS techniques, a database and data analysis application have been developed. Results: In silico developed database (~ 148000 gangliosides) considers the high diversity of ceramide compositions, sialic acid structures as well as possible modifications of standard ganglioside structures. Data analysis application enables correlation of MS and(or) low-energy tandem MS spectra with structures in the database. Tandem MS application simplifies tandem MS ion search by exploiting the fact that ganglioside fragmentation follows reverse biosynthetic pathway and accordingly uses MS ion database for matching tandem MS spectra. Validation conducted on GD1a standard as well as on complex mixture of native gangliosides isolated from human brain tissue demonstrated agreement with previous studies. Conclusion: Plain, fast and automated routine for characterization of gangliosides described here could facilitate and improve mass spectrometric analysis of complex mixtures in variety of tissues and pathological samples where it is known that distinctive changes in ganglioside composition occur.
Izvorni jezik
Engleski
Znanstvena područja
Kemija
POVEZANOST RADA
Projekti:
098-0982915-2945 - Spektroskopija, kemijska svojstva i reakcije biološki aktivnih molekula (Kovač, Branka, MZOS ) ( CroRIS)
108-1081870-2415 - Strukturno-funkcionalna glikolipidomika moždanog razvitka i maligne alteracije (Vukelić, Željka, MZOS ) ( CroRIS)
Ustanove:
Institut "Ruđer Bošković", Zagreb,
Medicinski fakultet, Zagreb