Naslov
Is paraoxonase genetic polymorphism related to myocardial infarction in Croatian population?

Autori
Ivanišević, Ana-Maria ; Topić, Elizabeta ; Štefanović, Mario ; Nikolić, Vjeran ; Čubrilo-Turek, Mirjana ; Juretić, Dubravka ; Rekić, Branka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Kongres hrvatskih biokemičara i molekularnih biologa uz međunarodno sudjelovanje, HB2000, Program i knjiga sažetaka / Floegel, Mirna - Zagreb : Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 2000, 86-86

Skup
Kongres hrvatskih biokemičara i molekularnih biologa

Mjesto i datum
Zagreb, Hrvatska, 13.10.2000. - 15.10.2000

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
paraoxonase; genetic polymorphism; myocardial infarction

Sažetak
Gene coding paraoxonase (PON1), a HDL associated enzyme, has been mapped to human chromosome 7 and is codominantly expressed as alleles A and G. The A allele codes for the aminoacid glutamine at codone 192 (A genotype) and the G allele for arginine (B genotype). This genetic polymorphism has been suggested to contribute to the development of atherosclerosis and coronary heart disease. However, data in the literature on the association of the A and B genotypes, coronary atherosclerosis and the occurrence of myocardial infarction is still controversial. We have therefore performed a study to evaluate the relationship between the PON1 polymorphism and the risk of myocardial infarction. PON1 genotypes were determined in 200 patients suffering from acute myocardial infarction (AMI) and in 200 randomly selected healthy volunteers, using the PCR-RFLP method by AlwI restriction enzyme digestion. Paraoxonase activity was assayed for AMI patients spectrophotomoetrically by the slightly modified method previously described by Mackness. A and B allelic frequencies were 71 for A and 29 for B allele both for patients and controls. Genotype frequencies differed significantly between patients and controls (P=0.008), with BB genotype being more common in patients. BB genotype was also more common in youger patients and in the low-risk group defined as non smokers having serum cholesterol <5.2mmol/L and Lp(a) concentrations <20mg/dL; the difference has not reached a statistical significance. Enzymatic activities differed significantly (P=0.004) between the patient group and previously measured activities for a control group (n=146). Our findings suggest that the paraoxonase BB genotype may represent an independent genetic risk factor for myocardial infarction.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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