Naslov
Conformation of DNA in low added salt solutions

Autori
Grgičin, Danijel ; Dolanski Babić, Sanja ; Ivek, Tomislav ; Vuletić, Tomislav ; Tomić, Silvia

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
7th Christmas Biophysics Workshop : Book of Abstracts / Pabst, Georg ; Heftberger, Peter ; Kollmitzer, Benjamin - Graz, 2012, 24-24

Skup
7th Christmas Biophysics Workshop (XBW 2012)

Mjesto i datum
Riegersburg, Austrija, 17.12.2012. - 18.12.2012

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
DNA; UV spectrophotometry; dielectric properties; dynamics and conformational changes; divalent counterions

Sažetak
Function of DNA in vivo is determined by its conformation, e.g. the transcription of DNA can not start if DNA is not in the single stranded (ss) form. We study influence of intrinsic DNA ions on conformation in low added salt limit (<0.02 mM). Although, this is an opposite limit, it is a step forward in understanding the DNA conformation in vivo. We use dielectric spectroscopy (DS) and UV spectrophotometry to study DNA semidilute solutions in the concentration range 0.01-5 g/L before and after the controlled denaturation. Denaturation was accomplished by heating the samples for 20 min at a temperature of 97°C, followed by quenching to 4°C. Measurements were subsequently made at 25°C. We focus on the question of minimal intrinsic concentration which keeps Mg-DNA in the native double stranded (ds) conformation. The results obtained are as follows. Mg-DNA is in ds conformation at least above the concentration c=0.01 g/L. Na-DNA is also in ds conformation, but below concentration c=0.5 g/L it shows locally exposed hydrophobic core in a dynamic sense [1]. [1] S.Tomić, S. Dolanski Babić, T. Vuletić, S. Krča, D.Ivanković, L. Griparić i R. Podgornik, Phys. Rev. E 75, 021905 (2007).

Izvorni jezik
Engleski

Znanstvena područja
Fizika

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