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Pregled bibliografske jedinice broj: 626498

Detection of Bovine viral diarrhea virus-specific neutralizing antibodies in fresh colostrum : A modification of the virus neutralization test

Bedeković, Tomislav; Mihaljević, Željko; Jungić, Andreja; Lemo, Nina; Lojkić, Ivana; Cvetnić, Željko; Čač, Željko
Detection of Bovine viral diarrhea virus-specific neutralizing antibodies in fresh colostrum : A modification of the virus neutralization test // Journal of veterinary diagnostic investigation, 25 (2013), 2; 263-266 doi:10.1177/1040638713478813 (međunarodna recenzija, članak, znanstveni)

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Naslov
Detection of Bovine viral diarrhea virus-specific neutralizing antibodies in fresh colostrum : A modification of the virus neutralization test

Autori
Bedeković, Tomislav ; Mihaljević, Željko ; Jungić, Andreja ; Lemo, Nina ; Lojkić, Ivana ; Cvetnić, Željko ; Čač, Željko

Izvornik
Journal of veterinary diagnostic investigation (1040-6387) 25 (2013), 2; 263-266

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Bovine viral diarrhea virus; fresh colostrum; specific antibodies; virus neutralization test

Sažetak
To eliminate cytotoxic effects of colostrum on cells, a modified virus neutralization test (VNT) for the detection of Bovine viral diarrhea virus- specific neutralizing antibodies in colostrum was developed. The new test was compared to the World Organization for Animal Health-recommended VNT and the results evaluated. The agreement of the new test compared to the standard VNT was determined to be 98%, whereas sensitivity and specificity of the modified VNT compared to the standard VNT were 100%. Bovine viral diarrhea virus-specific antibodies were detected in 42 sera samples and 38 colostrum samples. The antibody titers in serum and colostrum showed a high correlation (n = 56, r = 0.9719, P < 0.001). The modified virus neutralization technique described herein succeeds in eliminating cytotoxic effects and can be readily applied for the detection of specific antibodies against other infectious agents in colostrum.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina



POVEZANOST RADA

Projekti:
048-0481153-1127 - Istraživanje bolesti životinja s neurološkim poremećajima (Šoštarić, Branko, MZOS ) ( CroRIS)
048-0481153-1150 - Molekularna epizootiologija važnih bakterijskih zoonoza (Cvetnić, Željko, MZOS ) ( CroRIS)
048-0481186-1183 - Primjena genetičkih analiza u veterinarskoj medicini (Lojkić, Mirko, MZOS ) ( CroRIS)

Ustanove:
Hrvatski veterinarski institut, Zagreb

Profili:

Avatar Url Ivana Lojkić (autor)

Avatar Url Željko Čač (autor)

Avatar Url Tomislav Bedeković (autor)

Avatar Url Nina Krešić (autor)

Avatar Url Željko Cvetnić (autor)

Avatar Url Željko Mihaljević (autor)

Poveznice na cjeloviti tekst rada:

doi vdi.sagepub.com

Citiraj ovu publikaciju:

Bedeković, Tomislav; Mihaljević, Željko; Jungić, Andreja; Lemo, Nina; Lojkić, Ivana; Cvetnić, Željko; Čač, Željko
Detection of Bovine viral diarrhea virus-specific neutralizing antibodies in fresh colostrum : A modification of the virus neutralization test // Journal of veterinary diagnostic investigation, 25 (2013), 2; 263-266 doi:10.1177/1040638713478813 (međunarodna recenzija, članak, znanstveni)
Bedeković, T., Mihaljević, Ž., Jungić, A., Lemo, N., Lojkić, I., Cvetnić, Ž. & Čač, Ž. (2013) Detection of Bovine viral diarrhea virus-specific neutralizing antibodies in fresh colostrum : A modification of the virus neutralization test. Journal of veterinary diagnostic investigation, 25 (2), 263-266 doi:10.1177/1040638713478813.
@article{article, author = {Bedekovi\'{c}, Tomislav and Mihaljevi\'{c}, \v{Z}eljko and Jungi\'{c}, Andreja and Lemo, Nina and Lojki\'{c}, Ivana and Cvetni\'{c}, \v{Z}eljko and \v{C}a\v{c}, \v{Z}eljko}, year = {2013}, pages = {263-266}, DOI = {10.1177/1040638713478813}, keywords = {Bovine viral diarrhea virus, fresh colostrum, specific antibodies, virus neutralization test}, journal = {Journal of veterinary diagnostic investigation}, doi = {10.1177/1040638713478813}, volume = {25}, number = {2}, issn = {1040-6387}, title = {Detection of Bovine viral diarrhea virus-specific neutralizing antibodies in fresh colostrum : A modification of the virus neutralization test}, keyword = {Bovine viral diarrhea virus, fresh colostrum, specific antibodies, virus neutralization test} }
@article{article, author = {Bedekovi\'{c}, Tomislav and Mihaljevi\'{c}, \v{Z}eljko and Jungi\'{c}, Andreja and Lemo, Nina and Lojki\'{c}, Ivana and Cvetni\'{c}, \v{Z}eljko and \v{C}a\v{c}, \v{Z}eljko}, year = {2013}, pages = {263-266}, DOI = {10.1177/1040638713478813}, keywords = {Bovine viral diarrhea virus, fresh colostrum, specific antibodies, virus neutralization test}, journal = {Journal of veterinary diagnostic investigation}, doi = {10.1177/1040638713478813}, volume = {25}, number = {2}, issn = {1040-6387}, title = {Detection of Bovine viral diarrhea virus-specific neutralizing antibodies in fresh colostrum : A modification of the virus neutralization test}, keyword = {Bovine viral diarrhea virus, fresh colostrum, specific antibodies, virus neutralization test} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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