Naslov
Role of Rac1 GTPase in the regulation of Dictyostelium cell migration

Autori
Marinović, Maja ; Antolović, Vlatka, Filić, Vedrana ; Faix, Jan ; Weber, Igor

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Novel Biophysical Approaches in the Investigation of the Cytoskeleton ; The 27th European Cytoskeletal Forum Meeting / Nyitrai, Miklos ; Ampe, Christophe ; Machesky, Laura - Pečuh, 2012, 103-103

Skup
Novel Biophysical Approaches in the Investigation of the Cytoskeleton ; The 27th European Cytoskeletal Forum Meeting

Mjesto i datum
Pečuh, Mađarska, 03.11.2012. - 07.11.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Rac1 ; cell motility ; actin cytoskeleton

Sažetak
Rac proteins are the only canonical Rho family GTPases in Dictyostelium, where they act as key regulators of the actin cytoskeleton. We monitored the dynamics of activated Rac1 in Dictyostelium cells using fluorescent probes that specifically bind to GTP-bound form of Rac1. The probes were based on the GTPase-binding domain (GBD) from PAK1 kinase and the IQGAP-related protein DGAP1. In moving Dictyostelium cells, GBD probe was strongly enriched at the leading edge where it co-localized with F-actin, whereas DGAP1 probe was localized to the trailing, retracting regions of migrating cells. As assessed by latrunculin B treatment, cortical localization of PAK1_GBD strictly depends on the integrity of the actin cytoskeleton, whereas cortical localization of DGAP1 does not. Taken together, these results imply that Rac1 GTPases play a dual role, both at the front and in the back, in migrating Dictyostelium cells. In order to further characterize the regulation of cell migration by Rac1 proteins it is necessary to compare characteristics of cells with upregulated and downregulated GTPases in more detail. Whereas level of Rac1 GTPases is increased in Rac1A-overexpressing cells, cells with reduced level of these proteins will be generated using a multigene silencing approach. Using these cells, we plan to analyze localization and dynamics of the fluorescent probes in Rac1-enriched and Rac1-deprived backgrounds, and to compare migration behavior of these mutant cells within the framework of a generalized Langevin model of cell locomotion.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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