Naslov
Synthesis and application of gold nanoparticles to MDA-MB-231 breast cancer cells

Autori
Nikolina Hanžić

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad, diplomski

Fakultet
Prirodoslovno-matematički fakulet, Biološki odsjek, Molekularna biologija

Mjesto
Zagreb

Datum
13.02

Godina
2013

Stranica
44

Mentor
Gotić, Marijan

Ključne riječi
gold; citrate; glutathione; dextrane; cancer; radiosensitization

Sažetak
The aim of this work was to study the radiosensitization effect of three types of gold nanoparticles (GNPs) on MDA-MB-231 cancer cells in vitro. GNPs were synthesized in order to be coated with citrate, glutathione and amino-dextrane. Their size was determined using electron microscopy and UV-VIS spectrophotometer and was around 15 nm. GNPs were applied to a MDA-MB-231 cancer cell line and their accumulation in cells was investigated. Transmission electron microscopy demonstrated accumulation of GNPs in cytoplasmic vesicles, while the Mass Spectroscopy was used to demonstrate the highest uptake of amino-dextrane coated GNPs. Cells treated with GNPs were γ-irradiated and their radiosensitization effect was studied. Short-term MTT viability assay showed no cells sensitivity to the effect of ionizing radiation, whereas the long-term clonogenic assay showed high sensitivity to the cytotoxic effect of γ-irradiation. However, the high cytotoxicity of γ-irradiation alone to cancer cells did not allow the determination of the radiosensitization effect of synthesized GNPs.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

Napomena
The aim of this work was to study the radiosensitization effect of three types of gold nanoparticles (GNPs) on MDA-MB-231 cancer cells in vitro. GNPs were synthesized in order to be coated with citrate, glutathione and amino-dextrane. Their size was determined using electron microscopy and UV-VIS spectrophotometer and was around 15 nm. GNPs were applied to a MDA-MB-231 cancer cell line and their accumulation in cells was investigated. Transmission electron microscopy demonstrated accumulation of GNPs in cytoplasmic vesicles, while the Mass Spectroscopy was used to demonstrate the highest uptake of amino-dextrane coated GNPs. Cells treated with GNPs were γ-irradiated and their radiosensitization effect was studied. Short-term MTT viability assay showed no cells sensitivity to the effect of ionizing radiation, whereas the long-term clonogenic assay showed high sensitivity to the cytotoxic effect of γ-irradiation. However, the high cytotoxicity of γ-irradiation alone to cancer cells did not allow the determination of the radiosensitization effect of synthesized GNPs.

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