Pregled bibliografske jedinice broj: 624366
Adaptation of Aminoacyl-tRNA Synthetase Catalytic Core to Carrier Protein Aminoacylation
Adaptation of Aminoacyl-tRNA Synthetase Catalytic Core to Carrier Protein Aminoacylation // Structure, 21 (2013), 4; 614-626 doi:10.1016/j.str.2013.02.017 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 624366 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Adaptation of Aminoacyl-tRNA Synthetase Catalytic Core to Carrier Protein Aminoacylation
Autori
Močibob, Marko ; Ivić, Nives ; Luić, Marija ; Weygand- Đurašević, Ivana
Izvornik
Structure (0969-2126) 21
(2013), 4;
614-626
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
aaRS homologs ; amino acid:[carrier protein] ligase ; carrier protein ; noncanonical functions of aaRS ; seryl-tRNA synthetase
Sažetak
Amino acid:[carrier protein] ligases (aa:CP ligases) are newly discovered enzymes that are highly similar to class II aminoacyl-tRNA synthetases (aaRSs). However, while aaRSs aminoacylate tRNA and supply building blocks for ribosomal translation, aa:CP ligases transfer activated amino acids to the phosphopantetheine group of small carrier proteins. We have solved the crystal structure of an aa:CP ligase complexed with the carrier protein (CP). The CP prosthetic group enters the active site from a different direction than tRNA in class II aaRS complexes, through an idiosyncratic tunnel. CP binds to aa:CP ligase in fundamentally different manner compared to tRNA binding by structurally closely related aaRSs. Based on crystallographic analysis, an enzyme of altered CP specificity was designed, and the mechanism of amino acid transfer to the prosthetic group was proposed. The presented study reveals how conserved class II aaRS catalytic core can adapt to a novel function through minor structural alterations.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Biologija
POVEZANOST RADA
Projekti:
098-1191344-2943 - Protein-ligand međudjelovanja na atomnoj razini (Luić, Marija, MZOS ) ( CroRIS)
119-0982913-1358 - Strukturna raznolikost seril-tRNA sintetaza i točnost biosinteze proteina (Rokov Plavec, Jasmina; Weygand Đurašević, Ivana, MZOS ) ( CroRIS)
HRZZ-IS-09.01/293 - Nekanonske uloge aminoacil-tRNA-sintetaza
Ustanove:
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb
Profili:
Marija Luić
(autor)
Marko Močibob
(autor)
Ivana Weygand Đurašević
(autor)
Nives Ivić
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE