Naslov
Telomerase activity in breast cancer: Association wtih disease-free and ten-year survival

Autori
Dedić Plavetić, Natalija ; Kulić, Ana ; Sirotković- Skerlev, Maja ; Gamulin, Stjepan ; Jakić-Razumović, Jasminka ; Vrbanec, Jurica ; Vrbanec, Damir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the ..... ; u: The Breast 22 (2013) (S) / - , 2013, S67-S67

Skup
13th St Gallen International Breast Cancer Conference 2013.

Mjesto i datum
St. Gallen, Švicarska, 13.03.2013. - 16.03.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
telomerase activity; breast cancer; prognostic factor

Sažetak
BACKGROUND Telomerase activity contributes to cell immortality - a hallmark of cancer. Telomerase is a ribonucleoprotein complex that contains three subunits: catalytic subunit of human telomerase reverse transcriptase, telomerase RNA subunit and protein subunit. Increased telomerase activity has been detected in various tumors, including breast cancer. GOALS The aim of this study was to assess the telomerase activity in normal breast tissue, benign breast lesions and breast carcinoma and to compare the results. We also analyzed relationship between telomerase activity and disease-free survival and relationship with ten-year overall survival. MATERIALS AND METHODS We analyzed telomerase activity in the cytosol of 102 breast cancers, 40 benign breast lesions and 20 samples of normal breast tissue. Telomerase activity was assessed by TRAP assay (telomeric repeat amplification protocol) (Figure1). Cut-off value was defined as level of absorbance 0, 2 at 450 nm. RESULTS Telomerase activity was present in 77 (75, 49%) of 102 breast cancers . The absorbance range of positive breast carcinomas was 0, 202- 3, 459 with the median 1, 032. The absorbance range of negative breast carcinomas was 0, 021-0, 198 with the median 0, 0845. The range of telomerase activity in the cytosol of normal breast tissue was 0, 0170-0, 089 with the median 0, 053. In benign lesions the range of telomerase activity was 0, 0180-0, 144 with the median 0, 0789 (Figure 2). Telomerase activity in breast cancer was statistically significantly higher in comparison with those in benign lesions (p<0, 0001) and normal breast tissue (p=0, 0001) (Figure 2). We found statistically significantly higher telomerase activity in benign breast lesions compared with normal tissue (p=0, 0033). Kaplan- Meier analysis showed that patients with higher telomerase activity had shorter disease-free survival (p<0, 0001) and shorter ten-year overall survival (p<0, 0001) than those with lower telomerase activity (Figure 3 and Figure 4 respectively). CONCLUSION Our results suggest that assessment of telomerase activity may have prognostic value in patients with breast cancer.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA